An electrochemical DNA sensor for detection of cytokeratin 19

被引：3
|
作者：
Xu, Xiongwei [1 ]
Weng, Xiuhua [1 ,2 ]
Liu, Ailin [2 ]
Zhang, Xiaoxing [2 ]
Wang, Changlian [1 ]
Chen, Wei [2 ]
Lin, Xinhua [2 ]
机构：
[1] Fujian Med Univ, Affiliated Hosp 1, Dept Pharm, Fuzhou 350005, Peoples R China
[2] Fujian Med Univ, Dept Pharmaceut Anal, Fuzhou 350004, Peoples R China
来源：
ANALYTICAL METHODS | 2013年 / 5卷 / 09期
基金：
中国国家自然科学基金;
关键词：
PROBE CARRIER PLATFORM; HAIRPIN LNA PROBE; NUCLEIC-ACIDS; HYBRIDIZATION; BIOSENSOR; NANOPARTICLES; RECOGNITION; SURFACE; INDICATOR; ELECTRODE;
D O I：
10.1039/c3ay00037k
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
33258 as the hybridization indicator was reported. The biosensor was developed by using thiolated ssDNA as the capture probe for hybridization with CK19 gene on a gold electrode. A significant increase of the peak current for Hoechst 33258 upon hybridization of immobilized ssDNA with target DNA was observed. This peak current change was used to monitor the recognition of the CK19 DNA sequence. Immobilization and hybridization conditions determining the performance of the electrochemical assay were investigated. Under optimal conditions, the constructed electrochemical DNA biosensor has excellent specificity for single-base mismatch and complementary after hybridization. A detection limit of 1.8 x 10(-8) M for complementary target DNA can be
引用
收藏
页码：2329 / 2334
页数：6
相关论文
共 50 条
  • [1] Disposable DNA electrochemical sensor for hybridization detection
    Marrazza, G
    Chianella, I
    Mascini, M
    BIOSENSORS & BIOELECTRONICS, 1999, 14 (01): : 43 - 51
  • [2] Disposable DNA electrochemical sensor for hybridization detection
    Marrazza, G.
    Chianella, I.
    Mascini, M.
    Biosensors and Bioelectronics, 1999, 14 (01): : 43 - 51
  • [3] Electrochemical DNA sensor for Neisseria meningitidis detection
    Patel, Manoj K.
    Solanki, Pratima R.
    Kumar, Ashok
    Khare, Shashi
    Gupta, Sunil
    Malhotra, Bansi D.
    BIOSENSORS & BIOELECTRONICS, 2010, 25 (12): : 2586 - 2591
  • [4] NOVEL DNA SENSOR FOR ELECTROCHEMICAL GENE DETECTION
    HASHIMOTO, K
    ITO, K
    ISHIMORI, Y
    ANALYTICA CHIMICA ACTA, 1994, 286 (02) : 219 - 224
  • [5] Electrochemical DNA sensor for detection of single nucleotide polymorphisms
    Marques, LPJ
    Cavaco, I
    Pinheiro, JP
    Ribeiro, V
    Ferreira, GNM
    CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2003, 41 (04) : 475 - 481
  • [6] A Competitor-switched Electrochemical Sensor for Detection of DNA
    Fan Hao
    Xing Rong
    Xu Ying
    Chen Miao
    Wang Qingjiang
    He Pingang
    Fang Yuzhi
    CHINESE JOURNAL OF CHEMISTRY, 2010, 28 (10) : 1978 - 1982
  • [7] CtrA gene based electrochemical DNA sensor for detection of meningitis
    Patel, Manoj K.
    Solanki, Pratima R.
    Seth, Shruti
    Gupta, Sunil
    Khare, Shashi
    Kumar, Ashok
    Malhotra, B. D.
    ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (05) : 969 - 973
  • [8] An i-DNA based electrochemical sensor for proton detection
    Xu, Xiaodong
    Li, Bo
    Xie, Xiang
    Li, Xiaohui
    Shen, Li
    Shao, Yuanhua
    TALANTA, 2010, 82 (04) : 1122 - 1125
  • [9] Detection of Hepatitis B Virus DNA with a Paper Electrochemical Sensor
    Li, Xiang
    Scida, Karen
    Crooks, Richard M.
    ANALYTICAL CHEMISTRY, 2015, 87 (17) : 9009 - 9015
  • [10] Preparation of a novel DNA electrochemical sensor and its application to detection of DNA oxidative damage
    Wang, GX
    Pan, QX
    Wang, HS
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2005, 26 (10): : 1812 - 1816
12345