Effects of cold atmospheric pressure plasma and disinfecting agents onCandida albicansin root canals of extracted human teeth

被引:11
|
作者
Kerlikowski, Anne [1 ]
Matthes, Rutger [1 ]
Pink, Christiane [1 ]
Steffen, Heike [1 ]
Schluter, Rabea [2 ]
Holtfreter, Birte [1 ]
Weltmann, Klaus-Dieter [3 ]
von Woedtke, Thomas [3 ,4 ]
Kocher, Thomas [1 ]
Jablonowski, Lukasz [1 ]
机构
[1] Univ Med Greifswald, Dent Sch, Dept Restorat Dent Periodontol Endodontol Prevent, Rotgerberstr 8, D-17475 Greifswald, Germany
[2] Ernst Moritz Arndt Univ Greifswald, Dept Biol, Imaging Ctr, Greifswald, Germany
[3] Leibniz Inst Plasma Sci & Technol eV, Dept Plasma Life Sci, Greifswald, Germany
[4] Univ Med Greifswald, Dept Hyg & Environm Med, Greifswald, Germany
关键词
Candida albicans; cold atmospheric pressure plasma; human teeth; medical plasma; root canal; CANDIDA-ALBICANS; ENDODONTIC TREATMENT; IN-VITRO; INACTIVATION; BIOFILMS; THERAPY; FAECALIS;
D O I
10.1002/jbio.202000221
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reinfection in endodontically treated teeth is linked to the complexity of the root canal system, which is problematic to reach with conventional disinfection methods. As plasma is expected to have the ability to sanitize narrow areas, the aim of this study was to analyze the effect of cold atmospheric pressure plasma (CAP) onCandida albicansin root canals of extracted human teeth. CAP was applied as mono treatment and in combination with standard endodontic disinfectants (sodium hypochlorite, chlorhexidine and octenidine). Disinfection efficiency was evaluated as reduction of the logarithm of colony forming units per milliliter (log(10)CFU/mL) supported by scanning electron microscopy as imaging technique. Plasma alone showed the highest reduction of log(10)CFU, suggesting the best disinfection properties of all tested agents.
引用
下载
收藏
页数:9
相关论文
共 50 条
  • [21] Cold atmospheric pressure plasma treatment to assist the restoration of the apical region of a root canal in endodontic procedures
    Bisag, Alina
    Manzini, Michele
    Simoncelli, Emanuele
    Stancampiano, Augusto
    Tonini, Riccardo
    Gherardi, Matteo
    Colombo, Vittorio
    CLINICAL PLASMA MEDICINE, 2020, 19-20
  • [22] Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines
    Dezest, Marlene
    Chavatte, Laurent
    Bourdens, Marion
    Quinton, Damien
    Camus, Mylene
    Garrigues, Luc
    Descargues, Pascal
    Arbault, Stephane
    Burlet-Schiltz, Odile
    Casteilla, Louis
    Clement, Franck
    Planat, Valerie
    Bulteau, Anne-Laure
    SCIENTIFIC REPORTS, 2017, 7
  • [23] Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines
    Marlène Dezest
    Laurent Chavatte
    Marion Bourdens
    Damien Quinton
    Mylène Camus
    Luc Garrigues
    Pascal Descargues
    Stéphane Arbault
    Odile Burlet-Schiltz
    Louis Casteilla
    Franck Clément
    Valérie Planat
    Anne-Laure Bulteau
    Scientific Reports, 7
  • [24] Effects of atmospheric pressure cold plasma on human hepatocarcinoma cell and its 5-fluorouracil resistant cell line
    Yang, H.
    Lu, R.
    Xian, Y.
    Gan, L.
    Lu, X.
    Yang, X.
    PHYSICS OF PLASMAS, 2015, 22 (12)
  • [25] Selective Effects of Cold Atmospheric Plasma on Bone Sarcoma Cells and Human Osteoblasts
    Nitsch, Andreas
    Sieb, Konrad F.
    Qarqash, Sara
    Schoon, Janosch
    Ekkernkamp, Axel
    Wassilew, Georgi I.
    Niethard, Maya
    Haralambiev, Lyubomir
    BIOMEDICINES, 2023, 11 (02)
  • [26] Effects of different cold atmospheric-pressure plasma sources on the yeast Candida glabrata
    Trebulova, Kristina
    Krcma, Frantisek
    Skoumalova, Petra
    Kozakova, Zdenka
    Machala, Zdenko
    PLASMA PROCESSES AND POLYMERS, 2023, 20 (12)
  • [27] Direct and Indirect Bactericidal Effects of Cold Atmospheric-Pressure Microplasma and Plasma Jet
    Yahaya, Ahmad Guji
    Okuyama, Tomohiro
    Kristof, Jaroslav
    Blajan, Marius Gabriel
    Shimizu, Kazuo
    MOLECULES, 2021, 26 (09):
  • [28] Fluorescence microscopic analysis of antifungal effects of cold atmospheric pressure plasma in Saccharomyces cerevisiae
    Itooka, Koki
    Takahashi, Kazuo
    Izawa, Shingo
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (21) : 9295 - 9304
  • [29] Fluorescence microscopic analysis of antifungal effects of cold atmospheric pressure plasma in Saccharomyces cerevisiae
    Koki Itooka
    Kazuo Takahashi
    Shingo Izawa
    Applied Microbiology and Biotechnology, 2016, 100 : 9295 - 9304
  • [30] Atmospheric-Pressure Cold Plasma Induces Transcriptional Changes in Ex Vivo Human Corneas
    Rosani, Umberto
    Tarricone, Elena
    Venier, Paola
    Brun, Paola
    Deligianni, Velika
    Zuin, Matteo
    Martines, Emilio
    Leonardi, Andrea
    Brun, Paola
    PLOS ONE, 2015, 10 (07):