Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

被引：3

作者：

Lai, Meng Yee ^{[1
]}

Bukhari, Fatma Diyana Mohd ^{[1
]}

Zulkefli, Nur Zulaikha ^{[1
]}

Ismail, Ilyiana ^{[2
]}

Mustapa, Nur Izati ^{[2
]}

Soh, Tuan Suhaila Tuan ^{[2
]}

Hassan, Afifah Haji ^{[2
]}

Peariasamy, Kalaiarasu M. ^{[3
]}

Lee, Yee Leng ^{[4
]}

Suppiah, Jeyanthi ^{[5
]}

Thayan, Ravindran ^{[5
]}

Isa, Mohd Khairi Mat ^{[6
]}

Wahid, Nur Zafirah Abdul ^{[6
]}

Lau, Yee Ling ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Parasitol, Kuala Lumpur 50603, Malaysia

[2] Minist Hlth, Hosp Sungai Buloh, Dept Pathol, Kuala Lumpur, Malaysia

[3] Minist Hlth, Inst Clin Res, Natl Inst Hlth, Kuala Lumpur, Malaysia

[4] Minist Hlth, Clin Res Ctr, Hosp Sungai Buloh, Kuala Lumpur, Malaysia

[5] Minist Hlth, Infect Dis Res Ctr, Natl Inst Hlth, Virol Unit,Inst Med Res, Kuala Lumpur, Malaysia

[6] Selia Tek Holdings Sdn Bhd, Lot 18,Jalan Teknol 3-5, Kota Damansara 47810, Selangor, Malaysia

来源：

BMC INFECTIOUS DISEASES | 2022年 / 22卷 / 01期

关键词：

Diagnosis; RT-LAMP; SARS-CoV-2; Phenol red; pH sensitive indicator; VISUAL DETECTION;

D O I：

10.1186/s12879-022-07684-w

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. Methods A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. Results Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3-96.3%) and 93.6% specificity (95% CI 87.3-97.4%) of RT-LAMP. Conclusions The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool.

引用

页数：5

共 50 条

[21] Rapid and efficient detection of mycoplasma pneumonia RNA by reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
Ma, Cuiping
Yan, Chunyu
Shi, Chao
[J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255
[22] Detection of Equine Rotavirus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)
Nemoto, Manabu
Imagawa, Hiroshi
Tsujimura, Koji
Yamanaka, Takashi
Kondo, Takashi
Matsumura, Tomio
[J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 2010, 72 (06): : 823 - 826
[23] Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for point-of-care detection of SARS-CoV-2: a clinical study to evaluate agreement with RT-qPCR
Natoli, Mary
Kundrod, Kathryn
Chang, Megan
Smith, Chelsey
Paul, Sai
Eldin, Karen
Patel, Keyur
Baker, Ellen
Schmeler, Kathleen
Richards-Kortum, Rebecca
[J]. LANCET GLOBAL HEALTH, 2021, 9 : 3 - 3
[24] Applicability of a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for SARS-CoV-2 detection in high exposure risk setting
Nuchnoi, Pornlada
Piromtong, Pakorn
Siribal, Saranya
Anansilp, Korrarit
Thichanpiang, Peeradech
Okada, Pilailuk Akkapaiboon
[J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2023, 128 : 285 - 289
[25] RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) (vol 50, 2, 2022)
Lai, Meng Yee
Suppiah, Jeyanthi
Thayan, Ravindran
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Affah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Lau, Yee Ling
[J]. TROPICAL MEDICINE AND HEALTH, 2023, 51 (01)
[26] Rapid monitoring of SARS-CoV-2 on fruits and vegetables using reverse-transcription loop-mediated isothermal amplification assay
Dhanshetty, Manisha
Kusale, Supriya
Chavan, Vijayshree
Shewale, Shraddha
Lambe, Upendra P.
Sawant, Sonali A.
Nandi, Shyam Sundar
Saha, Sujoy
Holkar, Somnath
Jankar, Jagruti
Somkuwar, Ramhari
Banerjee, Kaushik
[J]. CURRENT SCIENCE, 2023, 124 (08): : 938 - 945
[27] A colorimetric reverse transcription-loop mediated isothermal amplification (RT-LAMP) method for the rapid detection of SARS-CoV-2 in Thailand
Bhakdeenuan, P.
Bunchoo, S.
Klayut, K.
Srisungngam, S.
Suphan, O.
Kongthap, I.
Suphankong, S.
Phetsuksiri, B.
Uppapong, B.
Rudeeaneksin, J.
[J]. TROPICAL BIOMEDICINE, 2024, 41 (01) : 64 - 69
[28] Development of Duplex and Multiplex Reverse Transcription Loop Mediated Isothermal Amplification (RT-LAMP) Assays for Clinical Diagnosis of SARS-COV-2 in Sri Lanka
Hewadikaram, M.
Perera, K.
Dissanayake, K.
Ramanayake, M.
Isurika, S. C.
Panch, A.
Jayarathne, A.
Pushpakumara, P.
Malavige, N.
Jeewandara, C.
Bathige, S. D. N. K.
Mubarak, A. M.
[J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2022, 116 : S39 - S40
[29] Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnostic
Nunes, Marcio R. T.
Vianez, Joau Lidio, Jr.
Nunes, Keley N. B.
da Silva, Sandro Patroca
Lima, Clayton P. S.
Guzman, Hilda
Martins, Livia C.
Carvalho, Valeria L.
Tesh, Robert B.
Vasconcelos, Pedro F. C.
[J]. JOURNAL OF VIROLOGICAL METHODS, 2015, 226 : 40 - 51
[30] Detection of human Enterovirus 71 reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Yaqing, H.
Wenping, Z.
Zhiyi, Y.
Xionghu, W.
Shouyi, Y.
Hong, Y.
Yingchun, D.
Guifang, H.
[J]. LETTERS IN APPLIED MICROBIOLOGY, 2012, 54 (03) : 233 - 239

← 1 2 3 4 5 →