Rapid monitoring of SARS-CoV-2 on fruits and vegetables using reverse-transcription loop-mediated isothermal amplification assay

被引:0
|
作者
Dhanshetty, Manisha [1 ]
Kusale, Supriya [1 ]
Chavan, Vijayshree [1 ]
Shewale, Shraddha [1 ]
Lambe, Upendra P. [2 ]
Sawant, Sonali A. [2 ]
Nandi, Shyam Sundar [2 ]
Saha, Sujoy [1 ]
Holkar, Somnath [1 ]
Jankar, Jagruti [1 ]
Somkuwar, Ramhari [1 ]
Banerjee, Kaushik [1 ]
机构
[1] ICAR Natl Res Ctr Grapes, Natl Reference Lab, PO Manjri Farm, Pune 412307, India
[2] Natl Inst Virol, Mumbai Unit, AD Marg, Mumbai 400012, India
来源
CURRENT SCIENCE | 2023年 / 124卷 / 08期
关键词
COVID-19; pandemic; fresh fruits and vege-tables; open markets and pack houses; packaging materials; transmission and monitoring;
D O I
10.18520/cs/v124/i8/938-945
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
COVID-infected people handling fruits and vegetables may spread the virus to healthy people on contact. Here we examined if SARS-CoV-2 was detectable on the fruits, vegetables, hand gloves and packaging materials collected from the open markets and pack houses in India. During the study (2021-22), swabs from 748 samples representing the majorly traded items were tested using RT-LAMP assay. The sensitivity and specificity of the kit were found to be equivalent to the RT-PCR assay. All test samples were found negative for SARS-CoV-2. Thus, it can be concluded that the virus is unlikely to spread to foods and packaging materials through human contact.
引用
收藏
页码:938 / 945
页数:8
相关论文
共 50 条
  • [1] A Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2
    Lu, Renfei
    Wu, Xiuming
    Wan, Zhenzhou
    Li, Yingxue
    Jin, Xia
    Zhang, Chiyu
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (08)
  • [2] Rapid and Extraction-Free Detection of SARS-CoV-2 from Saliva by Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification
    Lalli, Matthew A.
    Langmade, S. Joshua
    Chen, Xuhua
    Fronick, Catrina C.
    Sawyer, Christopher S.
    Burcea, Lauren C.
    Wilkinson, Michael N.
    Fulton, Robert S.
    Heinz, Michael
    Buchser, William J.
    Head, Richard D.
    Mitra, Robi D.
    Milbrandt, Jeffrey
    [J]. CLINICAL CHEMISTRY, 2021, 67 (02) : 415 - 424
  • [3] Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay
    Yan, C.
    Cui, J.
    Huang, L.
    Du, B.
    Chen, L.
    Xue, G.
    Li, S.
    Zhang, W.
    Zhao, L.
    Sun, Y.
    Yao, H.
    Li, N.
    Zhao, H.
    Feng, Y.
    Liu, S.
    Zhang, Q.
    Liu, D.
    Yuan, J.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2020, 26 (06) : 773 - 779
  • [4] A Multiplex and Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Sensitive and Rapid Detection of Novel SARS-CoV-2
    Juscamayta-Lopez, Eduardo
    Valdivia, Faviola
    Horna, Helen
    Tarazona, David
    Linares, Liza
    Rojas, Nancy
    Huaringa, Maribel
    [J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2021, 11
  • [5] Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA
    Zhang, Yinhua
    Tanner, Nathan A.
    [J]. BIOTECHNIQUES, 2021, 70 (03) : 167 - 174
  • [7] A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
    Lau, Yee
    Ismail, Ilyiana
    Mustapa, Nur
    Lai, Meng
    Soh, Tuan
    Hassan, Afifah
    Peariasamy, Kalaiarasu
    Lee, Yee
    Goh, Pik
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2020, 103 (06): : 2350 - 2352
  • [8] Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2
    Renfei Lu
    Xiuming Wu
    Zhenzhou Wan
    Yingxue Li
    Lulu Zuo
    Jianru Qin
    Xia Jin
    Chiyu Zhang
    [J]. Virologica Sinica, 2020, (03) : 344 - 347
  • [9] Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
    Lau, Yee Ling
    Ismail, Ilyiana
    Mustapa, Nur Izati
    Lai, Meng Yee
    Soh, Tuan Suhaila Tuan
    Hassan, Afifah
    Peariasamy, Kalaiarasu M.
    Lee, Yee Leng
    Chong, Yoong Min
    Sam, I-Ching
    Goh, Pik Pin
    [J]. PEERJ, 2020, 8
  • [10] Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2
    Lu, Renfei
    Wu, Xiuming
    Wan, Zhenzhou
    Li, Yingxue
    Zuo, Lulu
    Qin, Jianru
    Jin, Xia
    Zhang, Chiyu
    [J]. VIROLOGICA SINICA, 2020, 35 (03) : 344 - 347