A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2

被引：6
|
作者：
Lau, Yee [1 ]
Ismail, Ilyiana [2 ]
Mustapa, Nur [2 ]
Lai, Meng [1 ]
Soh, Tuan [2 ]
Hassan, Afifah [2 ]
Peariasamy, Kalaiarasu [3 ]
Lee, Yee [3 ]
Goh, Pik [4 ]
机构：
[1] Univ Malaya, Fac Med, Dept Parasitol, Jalan Lembah Pantai, Kuala Lumpur 50603, Malaysia
[2] Hosp Sungai Buloh, Dept Pathol, Sungai Buloh, Selangor, Malaysia
[3] Hosp Sungai Buloh, Clin Res Ctr, Sungai Buloh, Selangor, Malaysia
[4] Minist Hlth Malaysia, Natl Inst Hlth NIH, Inst Clin Res ICR, Putrajaya, Malaysia
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2020年 / 103卷 / 06期
关键词：
RAPID DETECTION;
D O I：
10.4269/ajtmh.20-1079
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
引用
收藏
页码：2350 / 2352
页数：3
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