A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization

被引：10
|
作者：
Ebrahimi, Maryam [1 ]
Raoof, Jahan Bakhsh [1 ]
Ojani, Reza [1 ]
Bagheryan, Zahra [1 ]
机构：
[1] Univ Mazandaran, Fac Chem, Dept Analyt Chem, Eletroanalyt Chem Res Lab, Babol Sar, Iran
来源：
ANALYTICAL BIOCHEMISTRY | 2015年 / 488卷
关键词：
Mercury ions biosensor; T-Hg2+-T complex; Silver nanoparticles; Ethyl green; DNA hybridization; HG2+;
D O I：
10.1016/j.ab.2015.07.011
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An electrochemical biosensor was developed for Hg2+ determination based on DNA hybridization. In the presence of Hg2+, the target and probe DNAs with thymine thymine (T-T) mismatches could hybridize by forming T-Hg2+-T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg2+. The difference in the value of the peak currents of EG before and after DNA hybridization (Delta I) was linear with the concentration of Hg2+ in the range of 9.0 x 10(-11)-1.0 x 10(-9) M. The detection limit was 3.08 x 10(-11) M. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：12 / 13
页数：2
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