A novel graphene-DNA biosensor for selective detection of mercury ions

被引:135
|
作者
Zhang, Yang [1 ]
Zhao, Hong [1 ]
Wu, Zhijiao [1 ]
Xue, Ying [1 ]
Zhang, Xiaofang [1 ]
He, Yujian [1 ]
Li, Xiangjun [1 ]
Yuan, Zhuobin [1 ]
机构
[1] Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China
来源
基金
中国国家自然科学基金;
关键词
Mercury ions; Graphene; Michael addition; Biosensor; Nucleic acids; FUNCTIONALIZED GOLD NANOPARTICLES; COLORIMETRIC DETECTION; AQUEOUS-MEDIA; BASE-PAIRS; HG-II; OXIDE; NANOSHEETS; HG(II); SENSITIVITY; MONOLAYERS;
D O I
10.1016/j.bios.2013.04.013
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A novel electrochemical biosensor for sensitive and selective detection of mercury (II) ions (Hg2+) based on a DNA grafted graphene is proposed. Graphene oxide (GO) was reduced by dopamine, and then the single-strand probe DNA modified at the 5'-end with an alkylamino modifier (NH2-ssDNA) was grafted on the reduced graphene oxide (RGO) surface via Michael addition reaction. In the presence of Hg2+, the target DNA with four thymine-thymine (T-T) mismatches would hybridize with the probe DNA on the glassy carbon electrode (GCE) through T-Hg2+-T coordination chemistry. The hybridization of the two oligonucleotides leads to the increase in the peak currents of [Ru(NH3)(6)](3+), which could be used for electrochemical sensing of Hg2+. The difference in the value of the peak currents of [Ru(NH3)(6)](3+) before and after DNA hybridization was linear with the concentration of Hg2+ in the range from 8.0 x 10(-9) to 1.0 x 10(-7) M with a linear coefficiency of 0.996. The detection limit was 5.0 x 10(-9) M (S/N=3). The proposed electrochemical biosensor is rapid, convenient and low-cost for effective sensing of Hg2+. Particularly, the proposed method was applied successfully to the determination of Hg2+ in real environmental samples. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:180 / 187
页数:8
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