A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization

被引：10

作者：

Ebrahimi, Maryam ^{[1
]}

Raoof, Jahan Bakhsh ^{[1
]}

Ojani, Reza ^{[1
]}

Bagheryan, Zahra ^{[1
]}

机构：

[1] Univ Mazandaran, Fac Chem, Dept Analyt Chem, Eletroanalyt Chem Res Lab, Babol Sar, Iran

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 488卷

关键词：

Mercury ions biosensor; T-Hg2+-T complex; Silver nanoparticles; Ethyl green; DNA hybridization; HG2+;

D O I：

10.1016/j.ab.2015.07.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrochemical biosensor was developed for Hg2+ determination based on DNA hybridization. In the presence of Hg2+, the target and probe DNAs with thymine thymine (T-T) mismatches could hybridize by forming T-Hg2+-T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg2+. The difference in the value of the peak currents of EG before and after DNA hybridization (Delta I) was linear with the concentration of Hg2+ in the range of 9.0 x 10(-11)-1.0 x 10(-9) M. The detection limit was 3.08 x 10(-11) M. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：12 / 13

页数：2

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