Spatial fluorescence cross-correlation spectroscopy

被引：23

作者：

Jaffiol, R ^{[1
]}

Blancquaert, Y ^{[1
]}

Delon, A ^{[1
]}

Derouard, J ^{[1
]}

机构：

[1] Univ Grenoble 1, Spectrometrie Phys Lab, UMR 5588, CNRS, F-38402 St Martin Dheres, France

来源：

APPLIED OPTICS | 2006年 / 45卷 / 06期

关键词：

D O I：

10.1364/AO.45.001225

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To improve the spatial discrimination between the two confocal volumes we propose filtering of fluorescence photocounts by rejecting the fluorescence background, which corresponds to particles located far from the center of the detection volumes. (c) 2006 Optical Society of America.

引用

页码：1225 / 1235

页数：11

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