Spatial fluorescence cross-correlation spectroscopy

被引：23

作者：

Jaffiol, R ^{[1
]}

Blancquaert, Y ^{[1
]}

Delon, A ^{[1
]}

Derouard, J ^{[1
]}

机构：

[1] Univ Grenoble 1, Spectrometrie Phys Lab, UMR 5588, CNRS, F-38402 St Martin Dheres, France

来源：

APPLIED OPTICS | 2006年 / 45卷 / 06期

关键词：

D O I：

10.1364/AO.45.001225

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To improve the spatial discrimination between the two confocal volumes we propose filtering of fluorescence photocounts by rejecting the fluorescence background, which corresponds to particles located far from the center of the detection volumes. (c) 2006 Optical Society of America.

引用

页码：1225 / 1235

页数：11

共 50 条

[31] Fluorescence cross-correlation spectroscopy using single wavelength laser excitation
Xie Chao
Dong Chao-Qing
Ren Ji-Cun
[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2008, 29 (05): : 897 - 901
[32] Practical guidelines for dual-color fluorescence cross-correlation spectroscopy
Bacia, Kirsten
Schwille, Petra
[J]. NATURE PROTOCOLS, 2007, 2 (11) : 2842 - 2856
[33] Unveiling mRNP composition by fluorescence correlation and cross-correlation spectroscopy using cell lysates
Mateu-Regue, Angels
Christiansen, Jan
Bagger, Frederik Otzen
Hellriegel, Christian
Nielsen, Finn Cilius
[J]. NUCLEIC ACIDS RESEARCH, 2021, 49 (20) : E119
[34] Dynamic assembly properties of nonmuscle myosin II isoforms revealed by combination of fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy
Mitsuhashi, Mariko
Sakata, Hiroshi
Kinjo, Masataka
Yazawa, Michio
Takahashi, Masayuki
[J]. JOURNAL OF BIOCHEMISTRY, 2011, 149 (03): : 253 - 263
[35] 'True' single-molecule molecule observations by fluorescence correlation spectroscopy and two-color fluorescence cross-correlation spectroscopy
Foeldes-Papp, Zeno
[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2007, 82 (02) : 147 - 155
[36] Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells
Ohrt, Thomas
Muetze, Joerg
Staroske, Wolfgang
Weinmann, Lasse
Hoeck, Julia
Crell, Karin
Meister, Gunter
Schwille, Petra
[J]. NUCLEIC ACIDS RESEARCH, 2008, 36 (20) : 6439 - 6449
[37] Monitoring helicase-catalyzed DNA unwinding by fluorescence anisotropy and fluorescence cross-correlation spectroscopy
Xi, Xu Guang
Deprez, Eric
[J]. METHODS, 2010, 51 (03) : 289 - 294
[38] Fluorescence Cross-Correlation Spectroscopy for time dependent flows: a numerical investigation.
Ceffa, Nicolo' Giovanni
Pozzi, Paolo
Bouzin, Margaux
Marquezin, Cassia Alessandra
Sironi, Laura
D'Alfonso, Laura
Collini, Maddalena
Chirico, Giuseppe
[J]. MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XIII, 2015, 9320
[39] Dual-color total internal reflection fluorescence cross-correlation spectroscopy
Leutenegger, Marcel
Blom, Hans
Widengren, Jerker
Eggeling, Christian
Goesch, Michael
Leitgeb, Rainer A.
Lasser, Theo
[J]. JOURNAL OF BIOMEDICAL OPTICS, 2006, 11 (04)
[40] Interaction dynamics of DNA octamer pairs studied by fluorescence cross-correlation spectroscopy
Hom, EFY
Verkman, AS
[J]. BIOPHYSICAL JOURNAL, 2001, 80 (01) : 359A - 359A

← 1 2 3 4 5 →