Mitotic spindles revisited - new insights from 3D electron microscopy

被引：14
|
作者：
Mueller-Reichert, Thomas [1 ]
Kiewisz, Robert [1 ]
Redemann, Stefanie [1 ,2 ,3 ]
机构：
[1] Tech Univ Dresden, Expt Ctr, Med Fac Carl Gustav Carus, Fiedlerstr 42, D-01307 Dresden, Germany
[2] Univ Virginia, Sch Med, Ctr Membrane & Cell Physiol, Charlottesville, VA 22908 USA
[3] Univ Virginia, Sch Med, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA
来源：
JOURNAL OF CELL SCIENCE | 2018年 / 131卷 / 03期
基金：
欧盟地平线“2020”;
关键词：
3D reconstruction; Electron tomography; Microtubule segmentation; Microtubule; Mitosis; Spindle; MICROTUBULE DYNAMICS; FLUORESCENCE MICROSCOPY; MOLECULAR SOCIOLOGY; CORRELATIVE LIGHT; IN-SITU; ELEGANS; TOMOGRAPHY; ORGANIZATION; PROTEIN; CELLS;
D O I：
10.1242/jcs.211383
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The mitotic spindle is a complex three-dimensional (3D) apparatus that functions to ensure the faithful segregation of chromosomes during cell division. Our current understanding of spindle architecture is mainly based on a plethora of information derived from light microscopy with rather few insights about spindle ultrastructure obtained from electron microscopy. In this Review, we will provide insights into the history of imaging of mitotic spindles and highlight recent technological advances in electron tomography and data processing, which have delivered detailed 3D reconstructions of mitotic spindles in the early embryo of the nematode Caenorhabditis elegans. Tomographic reconstructions provide novel views on spindles and will enable us to revisit and address long-standing questions in the field of mitosis.
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页数：8
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