Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy

被引：2

作者：

Chen, Jing ^{[2
]}

Hao, Hu ^{[1
]}

Guo, Guoqing ^{[3
]}

Li, Sitao ^{[1
]}

Xiao, Xin ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 6, Lab Inborn Errors Metab, Guangzhou 510655, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 6, Inst Gastroenterol, Guangzhou 510655, Guangdong, Peoples R China

[3] Jinan Univ, Coll Med, Dept Anat, Guangzhou 510632, Guangdong, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 07期

关键词：

atomic force microscopy; Rho-kinase; nerve cells; neurites; hippocampus; rats; neural regeneration; PROTEIN; GTPASES; INHIBITOR; DYNAMICS;

D O I：

10.3969/j.issn.1673-5374.2012.07.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons.

引用

页码：496 / 500

页数：5

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