Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy

被引：2
|
作者：
Chen, Jing [2 ]
Hao, Hu [1 ]
Guo, Guoqing [3 ]
Li, Sitao [1 ]
Xiao, Xin [1 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 6, Lab Inborn Errors Metab, Guangzhou 510655, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 6, Inst Gastroenterol, Guangzhou 510655, Guangdong, Peoples R China
[3] Jinan Univ, Coll Med, Dept Anat, Guangzhou 510632, Guangdong, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 07期
关键词：
atomic force microscopy; Rho-kinase; nerve cells; neurites; hippocampus; rats; neural regeneration; PROTEIN; GTPASES; INHIBITOR; DYNAMICS;
D O I：
10.3969/j.issn.1673-5374.2012.07.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons.
引用
收藏
页码：496 / 500
页数：5
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