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Protective effects of N-acetylcysteine in isoproterenol-induced myocardium injury in rats

被引：17
|
作者：
Liu, Bin [2 ]
Li, Weimin [2 ]
Li, Yue [2 ]
Wang, Zhonghua [3 ]
Li, Hongli [4 ]
Liu, Ping [5 ]
Fu, Jun [1 ]
机构：
[1] Harbin Med Coll, Clin Coll 1, Int Med Care Dept, Harbin 150001, Peoples R China
[2] Harbin Med Coll, Clin Coll 1, Cardiovasc Dept, Harbin 150001, Peoples R China
[3] Yangzhou Hongquan Hosp, Dept Cardiol, Yangzhou 225200, Peoples R China
[4] Shanghai Jiao Tong Univ, Dept Cardiol, Affiliated Peoples Hosp 1, Shanghai 200080, Peoples R China
[5] Harbin Med Coll, Dept Immunol, Harbin 150081, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2009年 / 36卷 / 04期
关键词：
Isoproterenol; N-acetylcysteine; Myocardium injury; Inflammatory factors; DNA-DAMAGE; CYSTEINE; INFARCTION; EXPRESSION; APOPTOSIS; CYTOKINE; CADMIUM; ALPHA; CELLS; MICE;
D O I：
10.1007/s11033-008-9241-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isoproterenol (ISO) has been found to cause severe injury in the myocardium. The aim of this study was to investigate the protective effects of N-acetylcysteine (NAC) on ISO-induced myocardial injury in rats and its underlying mechanisms. Fouty male Wistar rats were randomly divided into four groups: control, ISO, NAC, and ISO + NAC group. Myocardial histopathological observation were performed; The activities of creatine kinase isoenzyme-MB (CK-MB) and lactate dehydrogenase (LDH) were examined; Myocardium TNF-alpha and IL-1 beta gene expressions were examined by RT-PCR analysis; Myocardial expressions of TNF-alpha and IL-1 beta proteins were observed by immunohistochemical assay and western blotting analysis. The myocardial injury induced by ISO was significantly reduced by the treatment of NAC as judged by the reduction of myocardial necrosis. Compared with ISO group, rats pre-injected with NAC showed a significant decrease in the activities of cardiac marker enzymes such as CK-MB and LDH in serum. NAC inhibits the pro-inflammatory factors expressions (TNF-alpha and IL-1 beta) stimulated by ISO. In conclusion, NAC exerts significant cardio-protective effects against ISO-induced myocardial injury in rats, likely regulating pro-inflammatory factors expressions.
引用
收藏
页码：761 / 765
页数：5
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