Sense and nonsense of high-temperature liquid chromatography

被引：162

作者：

Heinisch, Sabine ^{[1
]}

Rocca, Jean-Louis ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, UMR 5180, Sci Analyt Lab, F-69622 Villeurbanne, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 04期

关键词：

High temperature; RP-HPLC; HTLC; UHPLC; Fast HPLC; Basic compounds; Pharmaceutical analysis; REVERSED-PHASE CHROMATOGRAPHY; SUBCRITICAL WATER CHROMATOGRAPHY; FLAME IONIZATION DETECTION; ESTIMATING DIFFUSION-COEFFICIENTS; POLYBUTADIENE-COATED ZIRCONIA; MASS-TRANSFER KINETICS; TANDEM COLUMN CONCEPT; ULTRA-HIGH-PRESSURE; MM ID COLUMNS; MOBILE-PHASE;

D O I：

10.1016/j.chroma.2008.11.079

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-temperature liquid chromatography (HTLC) is recognized today as a valuable technique in reversed-phase high-performance liquid chromatography (RP-HPLC). Column temperature can play a role in reducing analysis time, modifying retention, controlling selectivity, changing efficiency or improving detection sensitivity. The different effects of high temperatures on reversed-phase separations, the practical limitations due to the instrumentation, the lit-nits and the main advantages of HTLC, especially for the separation of polar and ionized compounds. are reviewed. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：642 / 658

页数：17

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