Sense and nonsense of high-temperature liquid chromatography

被引：162
|
作者：
Heinisch, Sabine [1 ]
Rocca, Jean-Louis [1 ]
机构：
[1] Univ Lyon 1, CNRS, UMR 5180, Sci Analyt Lab, F-69622 Villeurbanne, France
来源：
JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 04期
关键词：
High temperature; RP-HPLC; HTLC; UHPLC; Fast HPLC; Basic compounds; Pharmaceutical analysis; REVERSED-PHASE CHROMATOGRAPHY; SUBCRITICAL WATER CHROMATOGRAPHY; FLAME IONIZATION DETECTION; ESTIMATING DIFFUSION-COEFFICIENTS; POLYBUTADIENE-COATED ZIRCONIA; MASS-TRANSFER KINETICS; TANDEM COLUMN CONCEPT; ULTRA-HIGH-PRESSURE; MM ID COLUMNS; MOBILE-PHASE;
D O I：
10.1016/j.chroma.2008.11.079
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-temperature liquid chromatography (HTLC) is recognized today as a valuable technique in reversed-phase high-performance liquid chromatography (RP-HPLC). Column temperature can play a role in reducing analysis time, modifying retention, controlling selectivity, changing efficiency or improving detection sensitivity. The different effects of high temperatures on reversed-phase separations, the practical limitations due to the instrumentation, the lit-nits and the main advantages of HTLC, especially for the separation of polar and ionized compounds. are reviewed. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：642 / 658
页数：17
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