Liquid Chromatographic Method for Determination of Biogenic Amines in Imported Fish and Meat Products

Efficient liquid chromatographic method by employing o-phthaldehyde derivative for separation of biogenic amines e.g., (1) histamine, (2) tyramine, (3) tryptamine, (4) putrescine, (5) 2-phenylethylamine, (6) cadaverine, (7) 1,7-diaminoheptane in aqueous extract from imported fish and meat products available Iraq markets. The fish and meat samples were extracted by using 8 % trichloroacetic acid, the aqueous solution were purified by passing through disposable filter paper 0.45 mu m pore diameter. The aqueous solutions were pre-concentration using rotary evaporator reaching adjusted volume of 2 mL. 20 mu L was injected on HPLC ISC07/5/504Na (100 x 4.6 mm Id) column, mobile phase 0.6 N sodium citrate + 0.1 M boric acid, (pH 10) the eluted peak were detected by using pre-column o-phthaldehyde derivative. The concentrations of biogenic amines were determined by comparison the peak area of the sample with that of authentic standard. In this work, HPLC method was applied for measurements of most biogenic primary amine by using pre-colunm o-dialdehyde (OPA) derivative, to form high sensitive isoindole derivative of all amines. These derivatives gave low detection limit of 2 ng/mL. The results observed relatively high concentration of biogenic amine in imported fish and meats products due to manufacturer source and unsuitable storage method.