Determination of Chloramphenicol in Honey and Royal Jelly by Sample Clean-up with Molecular Imprinted Polymer

被引：0

作者：

Sakamoto, Yasuhiro ^{[1
]}

Saito, Koichi ^{[1
]}

Hata, Rikuko ^{[1
]}

Katabami, Shiori ^{[1
]}

Iwasaki, Yusuke ^{[1
]}

Ito, Rie ^{[1
]}

Nakazawa, Hiroyuki ^{[1
]}

机构：

[1] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan

来源：

BUNSEKI KAGAKU | 2012年 / 61卷 / 05期

关键词：

chloramphenicol; MIP; honey; royal jelly; TRACE ANALYSIS; LIQUID; RESIDUES; THIAMPHENICOL; FLORFENICOL;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sample clean-up method that employs a molecular imprinted polymer (MIP) was examined for potential use in the residual analysis of chloramphenicol (CAP) in honey and royal jelly. The clean-up effect of MIP was compared with that of conventional reversed-phase solid-phase extraction (SPE). Measurements for evaluating the clean-up efficiency were conducted by LC/UV, LC/MS or enzymed-linked immunosorbent assay (ELISA). LC/UV measurements of a sample after SPE yielded interference peaks originating from impurities on the chromatograms. The recovery was approximately 80 % for honey and 75 % for royal jelly. LC/MS measurements of a sample cleaned up with MIP showed no interference peaks due to the matrix effect. In addition, ELISA conducted on a sample cleaned up with MIP revealed that MIP suppressed interference by impurities in the antigen-antibody reaction. The developed sample clean-up method with MIP was able to remove impurities in dark honey and royal jelly, and is expected to be useful for the determination of residual CAP in live-stock products.

引用

页码：383 / 389

页数：7

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