Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA

被引：61

作者：

Weizmann, Y ^{[1
]}

Cheglakov, Z ^{[1
]}

Pavlov, V ^{[1
]}

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, Farkas Ctr Light Induced Proc, IL-91904 Jerusalem, Israel

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 14期

关键词：

DNA; enzymes; fluorescence; self-replication;

D O I：

10.1002/anie.200503810

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Fuel for a DNA machine: A Fokl enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)- and quencher (Q)-functionalized nucleic acid 2. Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6, which provides an optical output for the machine (see scheme). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2238 / 2242

页数：5

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