Preparation of peptide thioesters using Fmoc-solid-phase peptide synthesis and its application to the construction of a template-assembled synthetic protein (TASP)

被引：111

作者：

Futaki, S ^{[1
]}

Sogawa, K ^{[1
]}

Maruyama, J ^{[1
]}

Asahara, T ^{[1
]}

Niwa, M ^{[1
]}

Hojo, H ^{[1
]}

机构：

[1] OSAKA CITY UNIV,FAC ENGN,DEPT BIOAPPL CHEM,SUMIYOSHI KU,OSAKA 558,JAPAN

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 35期

关键词：

D O I：

10.1016/S0040-4039(97)01434-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Preparation of peptide thioesters was conducted through peptide chain construction with Fmoc-solid-phase peptide synthesis on a 2-chlorotrityl resin followed by coupling with HS-(CH2)(2)-COOEt and deprotection with 95% aqueous CF3COOH. The peptide thioester corresponding to a transmembrane segment of he calcium channel (S4 in repeat IV) thus obtained was introduced onto a peptide template to give an artificial four-helix-bundle protein. (C) 1997 Elsevier Science Ltd.

引用

页码：6237 / 6240

页数：4

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