Preparation of peptide thioesters using Fmoc-solid-phase peptide synthesis and its application to the construction of a template-assembled synthetic protein (TASP)

被引：111
|
作者：
Futaki, S [1 ]
Sogawa, K [1 ]
Maruyama, J [1 ]
Asahara, T [1 ]
Niwa, M [1 ]
Hojo, H [1 ]
机构：
[1] OSAKA CITY UNIV,FAC ENGN,DEPT BIOAPPL CHEM,SUMIYOSHI KU,OSAKA 558,JAPAN
来源：
TETRAHEDRON LETTERS | 1997年 / 38卷 / 35期
关键词：
D O I：
10.1016/S0040-4039(97)01434-2
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Preparation of peptide thioesters was conducted through peptide chain construction with Fmoc-solid-phase peptide synthesis on a 2-chlorotrityl resin followed by coupling with HS-(CH2)(2)-COOEt and deprotection with 95% aqueous CF3COOH. The peptide thioester corresponding to a transmembrane segment of he calcium channel (S4 in repeat IV) thus obtained was introduced onto a peptide template to give an artificial four-helix-bundle protein. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：6237 / 6240
页数：4
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