A sensitive, homogeneous fluorescence assay for detection of thymine DNA glycosylase activity based on exonuclease-mediated amplification

被引：30
|
作者：
Chen, Cuihua [1 ]
Zhou, Dianming [1 ]
Tang, Hao [1 ]
Liang, Manfen [1 ]
Jiang, Jianhui [1 ]
机构：
[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 52期
关键词：
EXCISION-REPAIR; N-GLYCOSYLASE; MAMMALIAN DNA; DEMETHYLATION; 5-METHYLCYTOSINE; CONVERSION; 5-CARBOXYLCYTOSINE; METHYLATION; DEAMINATION; TET1;
D O I：
10.1039/c3cc41700j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported.
引用
收藏
页码：5874 / 5876
页数：3
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