A sensitive, homogeneous fluorescence assay for detection of thymine DNA glycosylase activity based on exonuclease-mediated amplification

被引：30
|
作者：
Chen, Cuihua [1 ]
Zhou, Dianming [1 ]
Tang, Hao [1 ]
Liang, Manfen [1 ]
Jiang, Jianhui [1 ]
机构：
[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 52期
关键词：
EXCISION-REPAIR; N-GLYCOSYLASE; MAMMALIAN DNA; DEMETHYLATION; 5-METHYLCYTOSINE; CONVERSION; 5-CARBOXYLCYTOSINE; METHYLATION; DEAMINATION; TET1;
D O I：
10.1039/c3cc41700j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported.
引用
收藏
页码：5874 / 5876
页数：3
相关论文
共 50 条
  • [21] No Structure-Switching Required: A Generalizable Exonuclease-Mediated Aptamer-Based Assay for Small-Molecule Detection
    Canoura, Juan
    Wang, Zongwen
    Yu, Haixiang
    Alkhamis, Obtin
    Fu, Fengfu
    Xiao, Yi
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (31) : 9961 - 9971
  • [22] The dumbbell probe mediated triple cascade signal amplification strategy for sensitive and specific detection of uracil DNA glycosylase activity
    Yang, Yayun
    Liu, Xiaoting
    Zhang, Nan
    Jiang, Wei
    TALANTA, 2021, 234
  • [23] Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles
    Zhao, Han
    Yan, Ying
    Chen, Mingjian
    Hu, Tingting
    Wu, Kefeng
    Liu, Haisheng
    Ma, Changbei
    ANALYST, 2019, 144 (22) : 6689 - 6697
  • [24] Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification
    Zhao, Xu-Hua
    Ma, Qiu-Juan
    Wu, Xiang-Xiang
    Zhu, Xin
    ANALYTICA CHIMICA ACTA, 2012, 727 : 67 - 70
  • [25] A Highly Sensitive Electrochemical Platform for the Assay of Uracil-DNA Glycosylase Activity Combined with Enzymatic Amplification
    Hua Zhang
    Liangliang Zhang
    Jianhui Jiang
    Ruqin Yu
    Analytical Sciences, 2013, 29 : 193 - 198
  • [26] A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′-5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy
    Tian, Jing-Xuan
    Fang, Yan-Zhao
    Yang, Yi-Xuan
    Wu, Shuang
    Xiao, Qiang
    Kong, Xiang-Juan
    NEW JOURNAL OF CHEMISTRY, 2020, 44 (48) : 21211 - 21217
  • [27] A Highly Sensitive Electrochemical Platform for the Assay of Uracil-DNA Glycosylase Activity Combined with Enzymatic Amplification
    Zhang, Hua
    Zhang, Liangliang
    Jiang, Jianhui
    Yu, Ruqin
    ANALYTICAL SCIENCES, 2013, 29 (02) : 193 - 198
  • [28] Highly sensitive homogeneous electrochemical assay for methyltransferase activity based on methylation-responsive exonuclease III-assisted signal amplification
    Wang, Xiuzhong
    Liu, Xinglu
    Hou, Ting
    Li, Wei
    Li, Feng
    SENSORS AND ACTUATORS B-CHEMICAL, 2015, 208 : 575 - 580
  • [29] A unique dual recognition hairpin probe mediated fluorescence amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activities
    Wu, Yushu
    Yan, Ping
    Xu, Xiaowen
    Jiang, Wei
    ANALYST, 2016, 141 (05) : 1789 - 1795
  • [30] A sensitive strategy for the fluorescence detection of DNA methyltransferase activity based on the graphene oxide platform and T7 exonuclease-assisted cyclic signal amplification
    Ma, Yefei
    Chen, Lini
    Zhang, Liangliang
    Liao, Suqi
    Zhao, Jingjin
    ANALYST, 2015, 140 (12) : 4076 - 4082
12345