Micropropagation of Adiantum capillus-veneris L.

被引:0
|
作者
Vilchez, J. [1 ]
Albany, N. [2 ]
Martinez, L. [1 ]
Molina, M. [2 ]
Fernandez, C. [1 ]
机构
[1] Univ Zulia, Fac Agron, Dept Bot, AP 15205,4005ZU, Maracaibo 4011, Estado Zulia, Venezuela
[2] Univ Zulia, Fac Agron, Dept Quim, AP 15205,4005ZU, Maracaibo 4011, Estado Zulia, Venezuela
关键词
spore germination; culture system; in vitro multiplication;
D O I
暂无
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The commercial value of the fern Adiantum capillus-veneris L., encouraged to develop a micropropagation protocol; evaluating spore germination in three concentrations of sucrose (1.5, 3 and 4.5% m/v), the multiplication of sporophytes in 25 and 100% of (NH4)NO3 with 0 and 4.4 mu M 6 benzylaminopurine; also three culture systems were evaluated: gelled medium, liquid and temporary immersion (TI). Acclimatization was evaluated in the vermicompost (0, 5 and 10% v/v) in the substrate. 80.6% of spores germinated with 1.5% sucrose, followed by multiplication in 25% (NH4)NO3 with 9.51 g of fresh biomass, increasing to 10.71 g in TIS. All ferns acclimated with 5 and 10% of the vermicompost.
引用
收藏
页码:312 / 324
页数:13
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