The use of holographic interferometry and electron speckle pattern interferometry for diffusion measurement in biochemical and pharmaceutical engineering applications

被引：27

作者：

Axelsson, Anders ^{[1
]}

Marucci, Mariagrazia ^{[1
]}

机构：

[1] Lund Univ, LTH, Fac Engn, Dept Chem Engn, SE-22100 Lund, Sweden

来源：

OPTICS AND LASERS IN ENGINEERING | 2008年 / 46卷 / 12期

关键词：

ESPI; Holographic laser interferometry; Diffusion; Gel; Membrane; Protein; Pharmaceutical; Bioseparation; Osmosis;

D O I：

10.1016/j.optlaseng.2008.03.017

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

In this review holographic interferometry and electron speckle pattern interferometry are discussed as efficient techniques for diffusion measurements in biochemical and pharmaceutical applications. Transport phenomena can be studied, quantitatively and qualitatively, in gels, liquids and membranes. Detailed information on these phenomena is required to design effective chromatography bioseparation processes using gel beads or ultrafiltration membranes, and in the design of control led-release pharmaceuticals using membrane-coated pellets or tablets. The influence of gel concentration, ion strength in the liquid and the size of diffusing protein molecules can easily be studied with good accuracy. When studying membranes, the resistance can be quantified, and it is also possible to discriminate between permeable and semi-permeable membranes. In this review the influence of temperature, natural convection and light deflection on the accuracy of the diffusion measurements is also discussed. (c) 2008 Elsevier Ltd. All rights reserved.

引用

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页码：865 / 876

页数：12

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