A simple technique for long-term preservation of leptospires

被引：6
|
作者：
Samir, Ahmed [1 ]
Wasfy, Momtaz O. [2 ]
机构：
[1] Cairo Univ, Fac Vet Med, Dept Microbiol, Cairo, Egypt
[2] Naval Med Res Unit NAMRU 3, Cairo, Egypt
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2013年 / 53卷 / 03期
关键词：
Long-term; Preservation; Leptospira; LIQUID-NITROGEN; STORAGE; GROWTH;
D O I：
10.1002/jobm.201100551
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The viability of six serovars of Leptospira spp. was studied after long storage at 70 degrees C. The bacteria were either preserved in EllinghausenMcCulloughJohnsonHarris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark-ground microscopy over a period of 20 months at 70 degrees C. Addition of sheep blood was not significantly advantageous, since leptospires that were stored in EMJH showed a slight increase in number after recovery. The results suggest a very simple and useful technique for long-term preservation of such Leptospira.
引用
收藏
页码：299 / 301
页数：3
相关论文
共 50 条
  • [41] Long-term outcomes of spinal meningioma resection with outer layer of dura preservation technique
    Saiwai, Hirokazu
    Okada, Seiji
    Hayashida, Mitsumasa
    Harimaya, Katsumi
    Matsumoto, Yoshihiro
    Kawaguchi, Ken-ichi
    Iida, Kei-ichiro
    Kobayakawa, Kazu
    Yokota, Kazuya
    Maeda, Takeshi
    Tsuchiya, Kuniyoshi
    Arizono, Takeshi
    Saito, Taichi
    Nakaie, Kazutoshi
    Iwamoto, Yukihide
    Nakashima, Yasuharu
    [J]. JOURNAL OF CLINICAL NEUROSCIENCE, 2021, 83 : 68 - 70
  • [42] Histopathological evaluation and long-term results of soft tissue preservation technique in cholesteatoma surgery
    Hiraumi, Harukazu
    Kanemaru, Shin-ichi
    Miura, Makoto
    Yamamoto, Norio
    Sakamoto, Tatsunori
    Ito, Juichi
    [J]. EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2017, 274 (02) : 711 - 714
  • [43] NEW MODIFICATIONS IN CHOLINESTERASE TECHNIQUE FOR LONG-TERM PRESERVATION OF STAINING IN FROZEN SECTIONS OF PANCREAS
    Purwar, Rama Shanker
    [J]. ACTA MICROSCOPICA, 2012, 21 (02): : 84 - 99
  • [44] Histopathological evaluation and long-term results of soft tissue preservation technique in cholesteatoma surgery
    Harukazu Hiraumi
    Shin-ichi Kanemaru
    Makoto Miura
    Norio Yamamoto
    Tatsunori Sakamoto
    Juichi Ito
    [J]. European Archives of Oto-Rhino-Laryngology, 2017, 274 : 711 - 714
  • [45] SIMPLE TECHNIQUE FOR LONG-TERM CENTRAL VENOUS ACCESS IN THE PATIENT WITH THROMBOCYTOPENIC CARCINOMA
    RICCI, JL
    REINER, DS
    [J]. SURGERY GYNECOLOGY & OBSTETRICS, 1991, 172 (02): : 145 - 146
  • [46] Long-term preservation of leptospiras by liquid nitrogen
    Rossetti, C. A.
    Auteri, C. D.
    [J]. REVISTA ARGENTINA DE MICROBIOLOGIA, 2008, 40 (02): : 86 - 88
  • [47] Microperfusion techniques for long-term hypothermic preservation
    Ferrera, R
    Michel, P
    Hadour, G
    Chiari, P
    Chambers, D
    Rodriguez, C
    [J]. JOURNAL OF HEART AND LUNG TRANSPLANTATION, 2000, 19 (08): : 792 - 800
  • [48] EFFECTIVE SYSTEM FOR LONG-TERM ORGAN PRESERVATION
    WOODS, JE
    COOPERMAN, AM
    MATHEWS, LE
    [J]. MAYO CLINIC PROCEEDINGS, 1971, 46 (11) : 754 - +
  • [49] Standardisation in the area of digital long-term preservation
    Schrimpf, Sabine
    [J]. ARCHIVING 2008, FINAL PROGRAM AND PROCEEDINGS, 2008, : 62 - 65
  • [50] Investigation of long-term preservation of pancreatic islets
    杨华
    张秀辉
    [J]. Chinese Medical Journal, 1999, (02)
12345