A simple technique for long-term preservation of leptospires

被引：6
|
作者：
Samir, Ahmed [1 ]
Wasfy, Momtaz O. [2 ]
机构：
[1] Cairo Univ, Fac Vet Med, Dept Microbiol, Cairo, Egypt
[2] Naval Med Res Unit NAMRU 3, Cairo, Egypt
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2013年 / 53卷 / 03期
关键词：
Long-term; Preservation; Leptospira; LIQUID-NITROGEN; STORAGE; GROWTH;
D O I：
10.1002/jobm.201100551
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The viability of six serovars of Leptospira spp. was studied after long storage at 70 degrees C. The bacteria were either preserved in EllinghausenMcCulloughJohnsonHarris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark-ground microscopy over a period of 20 months at 70 degrees C. Addition of sheep blood was not significantly advantageous, since leptospires that were stored in EMJH showed a slight increase in number after recovery. The results suggest a very simple and useful technique for long-term preservation of such Leptospira.
引用
收藏
页码：299 / 301
页数：3
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