Determination of conductivity of bacteria by using cross-flow filtration

被引：1
|
作者：
Johansson, LE
Aldaeus, F
Jonsson, G
Hamp, S
Roeraade, J
机构：
[1] Malardalen Univ, Dept Biol & Chem Engn, SE-63105 Eskilstuna, Sweden
[2] Royal Inst Technol, Sch Chem Sci & Engn, SE-10044 Stockholm, Sweden
[3] Malardalen Univ, Dept Math & Phys, SE-72123 Vasteras, Sweden
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 08期
关键词：
Bacillus subtilis; conductivity; cross-flow filtration; isoconductance point;
D O I：
10.1007/s10529-006-0021-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An important property of the bacterial surface is its conductivity. To obtain reliable conductivity values, it is essential to handle the cells as gently as possible during the measurement procedure. We have developed a method where a standard conductivity meter is used in combination with cross-flow filtration, thus avoiding repeated centrifugation and resuspension. With this method, the conductivity of Bacillus subtilis was determined to be 7000 mu S/cm, which is a deviation from previously published data by almost an order of a magnitude.
引用
收藏
页码：601 / 603
页数：3
相关论文
共 50 条
  • [41] SET REALISTIC GOALS FOR CROSS-FLOW FILTRATION
    GYURE, DC
    CHEMICAL ENGINEERING PROGRESS, 1992, 88 (11) : 60 - 66
  • [42] HARVESTING RECOMBINANT MICROBIAL-CELLS USING CROSS-FLOW FILTRATION
    BAILEY, FJ
    WARF, RT
    MAIGETTER, RZ
    ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (09) : 647 - 652
  • [43] CROSS-FLOW FILTRATION OF SACCHAROMYCES-CEREVISIAE USING AN UNSTEADY JET
    MARANGES, C
    CASASNOVAS, C
    LAFFORGUEDELORME, C
    BIOTECHNOLOGY TECHNIQUES, 1995, 9 (09) : 649 - 654
  • [44] Stormwater particle removal using a cross-flow filtration and sedimentation device
    Schwarz, TS
    Wells, SA
    ADVANCES IN FILTRATION AND SEPARATION TECHNOLOGY, VOLS 13A AND 13B, 1999: ADVANCING FILTRATION AND SEPARATION SOLUTIONS FOR THE MILLENNIUM, 1999, : 219 - 226
  • [45] HIGH-CONCENTRATION CULTIVATION OF LACTIC-ACID BACTERIA IN FERMENTER WITH CROSS-FLOW FILTRATION
    TANIGUCHI, M
    KOTANI, N
    KOBAYASHI, T
    JOURNAL OF FERMENTATION TECHNOLOGY, 1987, 65 (02): : 179 - 184
  • [46] Law of substance separation on cross-flow filtration in turbulent flow
    Universitaet-GH Paderborn, Paderborn, Germany
    Chem Eng Technol, 1 (11-19):
  • [47] Law of substance separation on cross-flow filtration in turbulent flow
    Fritz, A
    Pahl, MH
    CHEMICAL ENGINEERING & TECHNOLOGY, 1996, 19 (01) : 11 - 19
  • [48] FILTRATION CHARACTERISTICS AND STRUCTURE OF CAKE IN CROSS-FLOW FILTRATION OF BACTERIAL SUSPENSION
    TANAKA, T
    ABE, K
    ASAKAWA, H
    YOSHIDA, H
    NAKANISHI, K
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1994, 78 (06): : 455 - 461
  • [49] Determination of Hydrodynamic Shear Force Exerted on Membrane Surface in Cross-Flow Filtration by Multiphase Flow Simulation
    Fang, Hsun-He
    Wu, Rome-Ming
    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 2008, 41 (10) : 961 - 966
  • [50] Municipal Wastewater Treatment Using a UASB Coupled with Cross-Flow Membrane Filtration
    An, YingYu
    Yang, FengLin
    Bucciali, Benjamin
    Wong, FookSin
    JOURNAL OF ENVIRONMENTAL ENGINEERING, 2009, 135 (02) : 86 - 91
12345