Determination of conductivity of bacteria by using cross-flow filtration

被引：1
|
作者：
Johansson, LE
Aldaeus, F
Jonsson, G
Hamp, S
Roeraade, J
机构：
[1] Malardalen Univ, Dept Biol & Chem Engn, SE-63105 Eskilstuna, Sweden
[2] Royal Inst Technol, Sch Chem Sci & Engn, SE-10044 Stockholm, Sweden
[3] Malardalen Univ, Dept Math & Phys, SE-72123 Vasteras, Sweden
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 08期
关键词：
Bacillus subtilis; conductivity; cross-flow filtration; isoconductance point;
D O I：
10.1007/s10529-006-0021-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An important property of the bacterial surface is its conductivity. To obtain reliable conductivity values, it is essential to handle the cells as gently as possible during the measurement procedure. We have developed a method where a standard conductivity meter is used in combination with cross-flow filtration, thus avoiding repeated centrifugation and resuspension. With this method, the conductivity of Bacillus subtilis was determined to be 7000 mu S/cm, which is a deviation from previously published data by almost an order of a magnitude.
引用
收藏
页码：601 / 603
页数：3
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