Magnetic Cu: CuO-GO nanocomposite for efficient dispersive micro-solid phase extraction of polycyclic aromatic hydrocarbons from vegetable, fruit, and environmental water samples by liquid chromatographic determination

In this research, we presented a magnetic dispersive micro-solid phase extraction (MD-mu-SPE) method coupled with high performance liquid chromatography (HPLC) based on the use of magnetic Cu: CuO-Graphene Oxide (GO) nanocomposite (Fe3O4/Cu: CuO/GO-NC) for the separation and preconcentration of polycyclic aromatic hydrocarbons (PAHs), i.e. naphthalene (Nap), phenanthrene (Phe), anthracene (Ant), and pyrene (Pyr), in vegetable (onion, tomato, carrot, herb, watermelon, lettuce, eggplant, and chili pepper), fruit (apple, watermelon, and grape), wastewater, and water samples. The MD-mu-SPE of PAHs in matrix samples was carried out, and the impacts of pH, ionic strength, extraction time, temperature, eluent volume, and sorbent mass on the recovery of PAHs were investigated by using Placket-Burman design (PBD). In addition, by using the central composite design (CCD), the best combination of each important variable was measured. Sorbent mass of 14 mg, eluent volume of 200 mu L, and 12 min extraction time at the central level of other factors were optimal conditions of pretreatment for the highest extraction recovery (ER%) of trace PAHs. Under the optimal conditions, the method proposed herein provided high enrichment factors ranged from 116.51 to 133.05, good linearity in the range of 10-3800 ng mL(-1) for Pyr, 3.0-3500 ng mL(-1) for Phe, 5.0-3200 ng mL(-1) for Nap, and 5.0-3000 ng mL(-1) for Ant with coefficient of determination (R-2) values between 0.9889 and 0.9963, low limits of detection (LOD) and quantification (LOQ) in the range of 0.015-0.061 and 0.485-2.034 ng mL(-1), respectively, and also satisfactory spiked recoveries (between 95.1% and 106.8%) with the relative standard deviations (RSDs) values in the range of 1.73%-5.62%. The Fe3O4/Cu: CuO/GO-NC-based MD-mu-SPE followed by HPLC-UV corroborated promising results for the convenient and effective determination of PAHs in the samples of vegetables, fruits, and environmental water. The results of this study revealed that our developed method is easy, feasible, precise, highly effective, and convenient to operate for the trace analysis of PAHs in different real samples. The extraction recovery was about 90% of the initial recovery after the sorbent usage for three times; therefore, the Fe3O4/Cu: CuO/GO-NC can readily be regenerated.