High-yield isolation of extracellular vesicles using aqueous two-phase system

被引：112

作者：

Shin, Hyunwoo ^{[1
]}

Han, Chungmin ^{[1
]}

Labuz, Joseph M. ^{[4
]}

Kim, Jiyoon ^{[2
]}

Kim, Jongmin ^{[1
]}

Cho, Siwoo ^{[1
]}

Gho, Yong Song ^{[3
]}

Takayama, Shuichi ^{[4
,5
]}

Park, Jaesung ^{[1
,2
]}

机构：

[1] POSTECH, Dept Mech Engn, Pohang 790784, Gyeongbuk, South Korea

[2] POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 790784, Gyeongbuk, South Korea

[3] POSTECH, Dept Life Sci, Pohang 790784, Gyeongbuk, South Korea

[4] Univ Michigan, Dept Biomed Engn, Coll Engn, Biointerfaces Inst, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Coll Engn, Macromol Sci & Engn Ctr, Biointerfaces Inst, Ann Arbor, MI 48109 USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

CELL-DERIVED EXOSOMES; INTERSTITIAL FLUID; SEPARATION; ANTIGEN; PROTEIN; MICROENVIRONMENT; BIOMARKERS; PARTITION; MICRORNA; LIQUID;

D O I：

10.1038/srep13103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (similar to 70%) in a short time (similar to 15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.

引用

页数：11

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