High-yield isolation of extracellular vesicles using aqueous two-phase system

被引：112

作者：

Shin, Hyunwoo ^{[1
]}

Han, Chungmin ^{[1
]}

Labuz, Joseph M. ^{[4
]}

Kim, Jiyoon ^{[2
]}

Kim, Jongmin ^{[1
]}

Cho, Siwoo ^{[1
]}

Gho, Yong Song ^{[3
]}

Takayama, Shuichi ^{[4
,5
]}

Park, Jaesung ^{[1
,2
]}

机构：

[1] POSTECH, Dept Mech Engn, Pohang 790784, Gyeongbuk, South Korea

[2] POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 790784, Gyeongbuk, South Korea

[3] POSTECH, Dept Life Sci, Pohang 790784, Gyeongbuk, South Korea

[4] Univ Michigan, Dept Biomed Engn, Coll Engn, Biointerfaces Inst, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Coll Engn, Macromol Sci & Engn Ctr, Biointerfaces Inst, Ann Arbor, MI 48109 USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

CELL-DERIVED EXOSOMES; INTERSTITIAL FLUID; SEPARATION; ANTIGEN; PROTEIN; MICROENVIRONMENT; BIOMARKERS; PARTITION; MICRORNA; LIQUID;

D O I：

10.1038/srep13103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (similar to 70%) in a short time (similar to 15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.

引用

页数：11

共 50 条

[31] High-Yield, Magnetic Harvesting of Extracellular Outer-Membrane Vesicles from Escherichia coli
Shi, Rui
Dong, Ziliang
Ma, Chongqing
Wu, Renfei
Lv, Rui
Liu, Sidi
Ren, Yijin
Liu, Zhuang
van der Mei, Henny C.
Busscher, Henk J.
Liu, Jian
SMALL, 2022, 18 (48)
[32] Isolation of papain from ripe papaya peel using aqueous two-phase extraction
Mohit Singla
Nandan Sit
Journal of Food Measurement and Characterization, 2023, 17 : 1685 - 1692
[33] Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation
Slyusarenko, Maria
Nikiforova, Nadezhda
Sidina, Elena
Nazarova, Inga
Egorov, Vladimir
Garmay, Yuri
Merdalimova, Anastasiia
Yevlampieva, Natalia
Gorin, Dmitry
Malek, Anastasia
POLYMERS, 2021, 13 (03) : 1 - 16
[34] Isolation of papain from ripe papaya peel using aqueous two-phase extraction
Singla, Mohit
Sit, Nandan
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2023, 17 (02) : 1685 - 1692
[35] New process design for plasmid isolation using aqueous two-phase systems.
Kula, MR
Frerix, A
Mueller, M
Hubbuch, J
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 229 : U183 - U184
[36] Technical Aspects of Separation Using Aqueous Two-Phase Systems in Enzyme Isolation Processes
Kroner, K. H.
Hustedt, H.
Granda, S.
Kula, M. -R.
BIOTECHNOLOGY AND BIOENGINEERING, 2009, 104 (02) : 218 - 239
[37] Analysis and separation of amyloid β-peptides using aqueous two-phase systems under stress conditions -: From aqueous two-phase system to liposome membrane system
Kuboi, Ryoichi
Umakoshi, Hiroshi
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN, 2006, 13 : 9 - 21
[38] Transformation of vesicles in aqueous two-phase system of an anionic gemini surfactant and a cationic conventional surfactant mixture
Jiang Rong
Huang Yi-Xiong
Zhao Jian-Xi
Huang Chang-Cang
CHINESE JOURNAL OF CHEMISTRY, 2008, 26 (04) : 635 - 639
[39] Isolation and purification of aloe anthraquinones based on an ionic liquid/salt aqueous two-phase system
Tan, Zhijian
Li, Fenfang
Xu, Xuelei
SEPARATION AND PURIFICATION TECHNOLOGY, 2012, 98 : 150 - 157
[40] Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
Santos, Juliana A.
Amaral, Marcel C. O.
Araujo, Thiago A. S.
Fernandes, Kessia G. C.
Chaves, Adilson C.
Morais, Marcia M. C.
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2007, 38 (01) : 62 - 64

← 1 2 3 4 5 →