High-yield isolation of extracellular vesicles using aqueous two-phase system

被引：112
|
作者：
Shin, Hyunwoo [1 ]
Han, Chungmin [1 ]
Labuz, Joseph M. [4 ]
Kim, Jiyoon [2 ]
Kim, Jongmin [1 ]
Cho, Siwoo [1 ]
Gho, Yong Song [3 ]
Takayama, Shuichi [4 ,5 ]
Park, Jaesung [1 ,2 ]
机构：
[1] POSTECH, Dept Mech Engn, Pohang 790784, Gyeongbuk, South Korea
[2] POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 790784, Gyeongbuk, South Korea
[3] POSTECH, Dept Life Sci, Pohang 790784, Gyeongbuk, South Korea
[4] Univ Michigan, Dept Biomed Engn, Coll Engn, Biointerfaces Inst, Ann Arbor, MI 48109 USA
[5] Univ Michigan, Coll Engn, Macromol Sci & Engn Ctr, Biointerfaces Inst, Ann Arbor, MI 48109 USA
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
新加坡国家研究基金会;
关键词：
CELL-DERIVED EXOSOMES; INTERSTITIAL FLUID; SEPARATION; ANTIGEN; PROTEIN; MICROENVIRONMENT; BIOMARKERS; PARTITION; MICRORNA; LIQUID;
D O I：
10.1038/srep13103
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (similar to 70%) in a short time (similar to 15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.
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收藏
页数：11
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