Description of Mediterraneibacter massiliensis, gen. nov., sp nov., a new genus isolated from the gut microbiota of an obese patient and reclassification of Ruminococcus faecis, Ruminococcus lactaris, Ruminococcus torques, Ruminococcus gnavus and Clostridium glycyrrhizinilyticum as Mediterraneibacter faecis comb. nov., Mediterraneibacter lactaris comb. nov., Mediterraneibacter torques comb. nov., Mediterraneibacter gnavus comb. nov and Mediterraneibacter glycyrrhizinilyticus comb. nov.

被引：47

作者：

Togo, Amadou Hamidou ^{[1
]}

Diop, Awa ^{[2
]}

Bittar, Fadi ^{[2
]}

Maraninchi, Marie ^{[3
]}

Valero, Rene ^{[3
,4
]}

Armstrong, Nicholas ^{[1
]}

Dubourg, Gregory ^{[1
]}

Labas, Noemie ^{[2
]}

Richez, Magali ^{[1
]}

Delerce, Jeremy ^{[1
]}

Levasseur, Anthony ^{[1
]}

Fournier, Pierre-Edouard ^{[2
]}

Raoult, Didier ^{[1
]}

Million, Matthieu ^{[1
]}

机构：

[1] Aix Marseille Univ, IRD, MEPHI, IHU Mediterranee Infect, 19-21 Blvd Jean Moulin, F-13005 Marseille, France

[2] Aix Marseille Univ, IRD, VITROME, IHU Mediterranee Infect, 19-21 Blvd Jean Moulin, F-13005 Marseille, France

[3] Aix Marseille Univ, INSERM1062, INRA1260, NORT, F-13385 Marseille, France

[4] CHU Hop Concept, APHM, Serv Nutr Malad Metabol & Endocrinol, F-13385 Marseille, France

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2018年 / 111卷 / 11期

关键词：

Mediterraneibacter massiliensis; Taxonogenomics; Culturomics; Gut microbiota; Obesity; DNA-DNA HYBRIDIZATION; HUMAN FECES; PHYLOGENETIC ANALYSIS; SPECIES DELINEATION; GENOME SEQUENCE; HIGH-THROUGHPUT; GASTRIC BYPASS; BACTERIA; TAXONOMY; PROKARYOTES;

D O I：

10.1007/s10482-018-1104-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An anaerobic isolate, strain AT7(T), was cultivated from a stool sample of a morbidly obese French woman using a microbial culturomics approach. The 16S rRNA gene sequence analysis showed that strain AT7(T) exhibited 96% nucleotide sequence similarity with Ruminococcus torques strain JCM 6553(T) (=ATCC 27756(T)=VPI B2-51(T)), currently the closest related species with a validly published name. The strain was observed to be a Gram-stain positive, non-motile, asporogenous and coccobacillary-shaped bacterium. It was found to be catalase positive and oxidase negative. Its major fatty acids were identified as C-16:0 (54%) and C-18:1n9 (30%). The draft genome of strain AT7(T) is 3,069,882bp long with 42.4% G+C content. 2925 genes were predicted, including 2867 protein-coding genes and 58 RNAs. Based on phenotypic, biochemical, phylogenetic and genomic evidence, we propose the creation of the new genus Mediterraneibacter and species, Mediterraneibacter massiliensis, that contains strain AT7(T) (=CSUR P2086(T)=DSM 100837(T)), and the reclassification of Ruminococcus faecis, Ruminococcus lactaris, Ruminococcus torques, Ruminococcus gnavus, Clostridium glycyrrhizinilyticum as Mediterraneibacter faecis comb. nov., with type strain Eg2(T) (=KCTC 5757(T)=JCM15917(T)), Mediterraneibacter lactaris comb. nov., with type strain ATCC 29176(T) (=VPI X6-29(T)), Mediterraneibacter torques comb. nov., with type strain ATCC 27756(T) (=VPI B2-51(T)), Mediterraneibacter gnavus comb. nov., with type strain ATCC 29149T (=VPI C7-9T) and Mediterraneibacter glycyrrhizinilyticus comb. nov., with type strain ZM35(T) (=JCM 13368(T)=DSM 17593(T)), respectively.

引用

页码：2107 / 2128

页数：22

共 50 条

[41] Reclassification of Methanogenium tationis and Methanogenium liminatans as Methanofollis tationis gen. nov., comb. nov. and Methanofollis liminatans comb. nov. and description of a new strain of Methanofollis liminatans
Zellner, G
Boone, DR
Keswani, J
Whitman, WB
Woese, CR
Hagelstein, A
Tindall, BJ
Stackebrandt, E
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 : 247 - 255
[42] Thalassobius mediterraneus gen. nov., sp nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov.
Arahal, DR
Macián, MC
Garay, E
Pujalte, MJ
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2005, 55 : 2371 - 2376
[43] Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb, nov., Raoultella terrigena comb. nov and Raoultella planticola comb. nov.
Drancourt, M
Bollet, C
Carta, A
Rousselier, P
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 : 925 - 932
[44] Reclassification of Gemmobacter changlensis to a new genus as Cereibacter changlensis gen. nov., comb. nov.
Suresh, G.
Sasikala, Ch.
Ramana, Ch. V.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2015, 65 : 794 - 798
[45] Reclassification of [Glaciecola] lipolytica and [Aestuariibacter] litoralis in Aliiglaciecola gen. nov., as Aliiglaciecola lipolytica comb. nov and Aliiglaciecola litoralis comb. nov., respectively
Jean, Wen Dar
Hsu, Cheng Yu
Huang, Ssu-Po
Chen, Jwo-Sheng
Lin, Saulwood
Su, Mong-Huai
Shieh, Wung Yang
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013, 63 : 2859 - 2864
[46] Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α-Proteobacteria:: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov, and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov.
Imhoff, JF
Petri, R
Süling, J
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 : 793 - 798
[47] Seimatosporium ignobilis comb. nov.
Vanev, SG
MYCOTAXON, 1998, 67 : 413 - 414
[48] Proposals of Curvibacter gracilis gen. nov., sp nov and Herbaspirillum putei sp nov for bacterial strains isolated from well water and reclassification of [Pseudomonas] huttiensis, [Pseudomonas] lanceolata, [Aquaspirillum] delicatum and [Aquaspirillum] autotrophicum as Herbaspirillum huttiense comb. nov., Curvibacter lanceolatus comb. nov., Curvibacter delicatus comb. nov and Herbaspirillum autotrophicum comb. nov.
Ding, LX
Yokota, A
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004, 54 : 2223 - 2230
[49] Acidimangrovimonas sediminis gen. nov., sp. nov., isolated from mangrove sediment and reclassification of Defluviimonas indica as Acidimangrovimonas indica comb. nov. and Defluviimonas pyrenivorans as Acidimangrovimonas pyrenivorans comb. nov.
Ren, Hao
Ma, Hui
Li, Hao
Huang, Lingfeng
Luo, Yuanrong
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2019, 69 (08) : 2445 - 2451
[50] Lophodermium eriophori comb. nov.
Johnston, PR
Scheuer, C
MYCOTAXON, 2003, 87 : 489 - 492

← 1 2 3 4 5 →