Acidimangrovimonas sediminis gen. nov., sp. nov., isolated from mangrove sediment and reclassification of Defluviimonas indica as Acidimangrovimonas indica comb. nov. and Defluviimonas pyrenivorans as Acidimangrovimonas pyrenivorans comb. nov.

A Gram-stain-negative, aerobic, non-motile, short-rod bacterium, strain MS2-2(T), was isolated from mangrove sediment sampled at Jiulong River Estuary, Fujian province, PR China. 16S rRNA gene sequence similarity analysis showed that strain MS2-2(T) was most closely related to Defluviimonas indica 20V17(T) (97.41 %) and Defluviimonas pyrenivorans PrR001 T (96.18 %). Phylogenetic trees based on 16S rRNA genes and genome sequences both revealed that strain MS2-2(T) formed a distinct cluster with a indica 20V17(T) and a pyrenivorans PrR001 T within family Rhodobacteracea, quite separate from other type species in the genus Defluviimonas. The average nucleotide identity value between strain MS2-2(T) and a indica 20V17(T) was 78.35 %. Growth of strain MS2-2(T) was observed at 16-41 degrees C (optimum, 34 degrees C), pH 3.6-7.5 (pH 6.0) and 0.5-10.0 % (w/v) NaCl (4.0%). The major cellular fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), C-16:0 and C-18:0. Ubiquinone 10 was the sole quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 67.9 mol%. The combined genotypic and phenotypic data show that strain MS2-2(T) represents a novel species of a novel genus in the family Rhodobacteraceae, for which the name Acidimangrovimonassediminis gen. nov., sp. nov. is proposed, with the type strain MS2-2(T) (=MCCC 1K02682(T)=NBRC 112978(T)). We also propose the reclassification of Defluviimonas indica as Acidimangrovimonas indica comb. nov. and Defluviimonas pyrenivorans as Acidimangrovimonas pyrenivorans comb. nov.