Locus-specific chromatin isolation

被引：13
|
作者：
Vermeulen, Michiel [1 ]
Dejardin, Jerome [2 ]
机构：
[1] Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Oncode Inst, Dept Mol Biol, Nijmegen, Netherlands
[2] Univ Montpellier, Inst Human Genet, CNRS, Montpellier, France
来源：
NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2020年 / 21卷 / 05期
关键词：
CAPTURE;
D O I：
10.1038/s41580-020-0217-0
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Identifying factors bound to specific genomic loci in an unbiased manner is the holy grail of chromatin research, because it would provide an ultimate description of locus function. In this Comment, we explain why purifying a single locus represents a major technical challenge and provide recommendations for achieving this goal. Michiel Vermeulen and Jerome Dejardin recommend how best to characterize the proteins that bind any specific genomic locus.
引用
收藏
页码：249 / 250
页数：2
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