Locus-specific chromatin isolation

被引：13
|
作者：
Vermeulen, Michiel [1 ]
Dejardin, Jerome [2 ]
机构：
[1] Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Oncode Inst, Dept Mol Biol, Nijmegen, Netherlands
[2] Univ Montpellier, Inst Human Genet, CNRS, Montpellier, France
来源：
NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2020年 / 21卷 / 05期
关键词：
CAPTURE;
D O I：
10.1038/s41580-020-0217-0
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Identifying factors bound to specific genomic loci in an unbiased manner is the holy grail of chromatin research, because it would provide an ultimate description of locus function. In this Comment, we explain why purifying a single locus represents a major technical challenge and provide recommendations for achieving this goal. Michiel Vermeulen and Jerome Dejardin recommend how best to characterize the proteins that bind any specific genomic locus.
引用
收藏
页码：249 / 250
页数：2
相关论文
共 50 条
  • [21] Locus-specific chromatin proteomics using dCas-guided proximity labelling in Aspergillus nidulans
    Svoboda, Thomas
    Niederdoeckl-Loibl, Dominik
    Schueller, Andreas
    Hummel, Karin
    Schlosser, Sarah
    Razzazi-Fazeli, Ebrahim
    Strauss, Joseph
    FUNGAL GENETICS AND BIOLOGY, 2025, 178
  • [22] In vivo locus-specific editing of the neuroepigenome
    Yun Young Yim
    Collin D. Teague
    Eric J. Nestler
    Nature Reviews Neuroscience, 2020, 21 : 471 - 484
  • [23] A survey of locus-specific database curation
    Cotton, Richard G. H.
    Phillips, Kate
    Horaitis, Ourania
    JOURNAL OF MEDICAL GENETICS, 2007, 44 (04)
  • [24] In vivo locus-specific editing of the neuroepigenome
    Yim, Yun Young
    Teague, Collin D.
    Nestler, Eric J.
    NATURE REVIEWS NEUROSCIENCE, 2020, 21 (09) : 471 - 484
  • [25] ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation
    Lavinia Bisceglie
    Giody Bartolomei
    Michael O Hottiger
    Nature Protocols, 2017, 12 : 1951 - 1961
  • [26] ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation
    Bisceglie, Lavinia
    Bartolomei, Giody
    Hottiger, Michael O.
    NATURE PROTOCOLS, 2017, 12 (09) : 1951 - 1961
  • [27] Locus-specific microsatellite markers in common bean (Phaseolus vulgaris L.): isolation and characterization
    B. L’taief
    R. Horres
    R. Jungmann
    C. Molina
    B. Sifi
    M. Lachaâl
    P. Winter
    G. Kahl
    Euphytica, 2008, 162 (2) : 311 - 312
  • [28] Locus-specific microsatellite markers in common bean (Phaseolus vulgaris L.): isolation and characterization
    B. L’taief
    R. Horres
    R. Jungmann
    C. Molina
    B. Sifi
    M. Lachaâl
    P. Winter
    G. Kahl
    Euphytica, 2008, 162 : 301 - 310
  • [29] Locus-specific gene repositioning in prostate cancer
    Leshner, Marc
    Devine, Michelle
    Roloff, Gregory W.
    True, Lawrence D.
    Misteli, Tom
    Meaburn, Karen J.
    MOLECULAR BIOLOGY OF THE CELL, 2016, 27 (02) : 236 - 246
  • [30] LOCUS-SPECIFIC TRANSCRIPTIONAL CONTROL OF HLA GENES
    SOONG, TW
    HUI, KM
    JOURNAL OF IMMUNOLOGY, 1992, 149 (06): : 2008 - 2020
12345