Multiplexed evaluation of mouse wound tissue using oligonucleotide barcoding with single-cell RNA sequencing

被引：0

作者：

Januszyk, Michael ^{[1
]}

Griffin, Michelle ^{[1
]}

Mascharak, Shamik ^{[1
]}

Talbott, Heather E. ^{[1
]}

Chen, Kellen ^{[1
]}

Henn, Dominic ^{[1
]}

Spielman, Amanda F. ^{[1
]}

Parker, Jennifer B. L. ^{[1
]}

Liang, Norah E. ^{[1
]}

Cotterell, Asha ^{[1
]}

Guardino, Nicholas ^{[1
]}

Foster, Deshka S. ^{[1
]}

Wagh, Dhananjay ^{[2
]}

Coller, John ^{[2
]}

Gurtner, Geoffrey C. ^{[1
]}

Wan, Derrick C. ^{[1
]}

Longaker, Michael T. ^{[1
]}

机构：

[1] Stanford Univ, Hagey Lab Pediat Regenerat Med, Div Plast & Reconstruct Surg, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Stanford Genom Facil, Stanford, CA 94305 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Gene Expression; Model Organisms; RNAseq; Single Cell;

D O I：

10.1016/j.xpro.2022.101946

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite its rapidly increased availability for the study of complex tissue, single cell RNA sequencing remains prohibitively expensive for large studies. Here, we present a protocol using oligonucleotide barcoding for the tagging and pooling of multiple samples from healing wounds, which are among the most challenging tissue types for this application. We describe steps to generate skin wounds in mice, followed by tissue harvest and oligonucleotide barcoding. This protocol is also applicable to other species including rats, pigs, and humans.For complete details on the use and execution of this protocol, please refer to Stoeckius et al. (2018),1 Galiano et al. (2004),2 and Mascharak et al. (2022).3

引用

页数：12

共 50 条

[31] Single-cell RNA Sequencing in Immunology
Cao, Yirui
Qiu, Yue
Tu, Guowei
Yang, Cheng
CURRENT GENOMICS, 2020, 21 (08) : 564 - 575
[32] Multiplexed Characterization of AAV Cellular Tropism and Promoter Expression in Large Animals Using Single-Cell RNA Sequencing
Araki, Alex
Jensen, Martin Borch
Bowman, Chase
Carrico, Chris
Chio, Linda
Dixit, Atray
Duwaerts, Caroline
Fuentes, Daniel
Driver, Ian
Kartha, Vinay
Kuwahara, Akela
Le, Carmen
LePort, Francisco
Miyazaki, Hikaru
Morgan, Wyatt
Popov, Dimitry
Sachse, Frank
Sahu, Neety
Shambhu, Smitha
Towne, Christopher
Levings, Rachael Watson
MOLECULAR THERAPY, 2023, 31 (04) : 9 - 10
[33] Single-cell RNA sequencing in osteoarthritis
Gu, Yuyuan
Hu, Yan
Zhang, Hao
Wang, Sicheng
Xu, Ke
Su, Jiacan
CELL PROLIFERATION, 2023, 56 (12)
[34] Predicting Algorithm of Tissue Cell Ratio Based on Deep Learning Using Single-Cell RNA Sequencing
Liu, Zhendong
Lv, Xinrong
Chen, Xi
Li, Dongyan
Qin, Mengying
Bai, Ke
Yang, Yurong
Li, Xiaofeng
Zhang, Peng
APPLIED SCIENCES-BASEL, 2022, 12 (12):
[35] Preparing Highly Viable Single-Cell Suspensions from Mouse Pancreatic Islets for Single-Cell RNA Sequencing
Lee, Hugo
Engin, Feyza
STAR PROTOCOLS, 2020, 1 (03):
[36] Ultra-high throughput single-cell TCR and transcriptome sequencing using combinatorial barcoding RNA-seq
Song, Z.
Tan, L.
Prinz, I.
EUROPEAN JOURNAL OF IMMUNOLOGY, 2022, 52 : 405 - 405
[37] Construction of the Single-Cell Landscape of Hashimoto's Thyroiditis Tissue and Peripheral Blood by Single-Cell RNA Sequencing
Song, Kaiyu
Wang, Xiaojie
Yao, Wenjie
Wang, Yuantao
Zhang, Qinling
Tang, Yuxiao
Mou, Yakui
Song, Xicheng
Zhou, Jin
IMMUNITY INFLAMMATION AND DISEASE, 2025, 13 (02)
[38] Dissecting Cellular Heterogeneity Using Single-Cell RNA Sequencing
Choi, Yoon Ha
Kim, Jong Kyoung
MOLECULES AND CELLS, 2019, 42 (03) : 189 - 199
[39] Multiplexed Droplet Single-Cell RNA-Sequencing for Cellular Profiling of Transplant Rejection.
Schroeder, A.
Sigdel, T.
Damm, L.
Sur, S.
Hartoularos, G.
Ye, J.
Sarwal, M.
AMERICAN JOURNAL OF TRANSPLANTATION, 2019, 19 : 595 - 595
[40] Characterization of germ cell differentiation in the male mouse through single-cell RNA sequencing
S. Lukassen
E. Bosch
A. B. Ekici
A. Winterpacht
Scientific Reports, 8

← 1 2 3 4 5 →