Multiplexed evaluation of mouse wound tissue using oligonucleotide barcoding with single-cell RNA sequencing

被引：0

作者：

Januszyk, Michael ^{[1
]}

Griffin, Michelle ^{[1
]}

Mascharak, Shamik ^{[1
]}

Talbott, Heather E. ^{[1
]}

Chen, Kellen ^{[1
]}

Henn, Dominic ^{[1
]}

Spielman, Amanda F. ^{[1
]}

Parker, Jennifer B. L. ^{[1
]}

Liang, Norah E. ^{[1
]}

Cotterell, Asha ^{[1
]}

Guardino, Nicholas ^{[1
]}

Foster, Deshka S. ^{[1
]}

Wagh, Dhananjay ^{[2
]}

Coller, John ^{[2
]}

Gurtner, Geoffrey C. ^{[1
]}

Wan, Derrick C. ^{[1
]}

Longaker, Michael T. ^{[1
]}

机构：

[1] Stanford Univ, Hagey Lab Pediat Regenerat Med, Div Plast & Reconstruct Surg, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Stanford Genom Facil, Stanford, CA 94305 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Gene Expression; Model Organisms; RNAseq; Single Cell;

D O I：

10.1016/j.xpro.2022.101946

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite its rapidly increased availability for the study of complex tissue, single cell RNA sequencing remains prohibitively expensive for large studies. Here, we present a protocol using oligonucleotide barcoding for the tagging and pooling of multiple samples from healing wounds, which are among the most challenging tissue types for this application. We describe steps to generate skin wounds in mice, followed by tissue harvest and oligonucleotide barcoding. This protocol is also applicable to other species including rats, pigs, and humans.For complete details on the use and execution of this protocol, please refer to Stoeckius et al. (2018),1 Galiano et al. (2004),2 and Mascharak et al. (2022).3

引用

页数：12

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