Protocol for isolation of signet ring cells from human gastric mucosa

被引：0
|
作者：
Samaranayake, Sarah G. [1 ]
Gamble, Lauren A. [1 ]
Bowden, Cassidy [1 ]
Green, Benjamin L. [1 ]
Gallanis, Amber F. [1 ]
Akbulut, Dilara [2 ]
Shah, Niharika [2 ]
Hernandez, Jonathan M. [1 ]
Davis, Jeremy L. [1 ]
机构：
[1] NCI, Surg Oncol Program, NIH, Bethesda, MD 20892 USA
[2] NCI, Lab Pathol, NIH, Bethesda, MD 20892 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金：
美国国家卫生研究院;
关键词：
CANCER ORGANOIDS;
D O I：
10.1016/j.xpro.2023.102695
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric tissue. We describe the steps for gastric mucosa isolation and tissue dissociation. We then detail procedures for embedding cells into HistoGel for immunohistochemistry staining and additional applications such as flow cytometry and single-cell sequencing.
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收藏
页数：14
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