Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells

被引：5
|
作者：
Geng, Lihua [1 ,2 ,3 ,4 ]
Zhang, Quanbin [1 ,2 ,3 ,4 ]
Li, Qiong [5 ]
Zhang, Qian [4 ,6 ]
Wang, Cong [7 ]
Song, Ni [7 ]
Xin, Wenyu [8 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
[2] Inst Oceanol, Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
[3] Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
[4] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
[5] Shandong Marine Forecast & Hazard Mitigat Serv, Qingdao, Peoples R China
[6] Chinese Acad Sci, Inst Oceanol, Publ Technol Serv Ctr, Qingdao, Peoples R China
[7] Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China
[8] Binzhou Med Univ, Sch Pharm, Key Lab Prescript Effect & Clin Evaluat State Adm, Yantai, Shandong, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2024年 / 332卷
基金：
中国国家自然科学基金;
关键词：
Silvetia siliquosa; Fucoidan; Polysaccharide; Immunomodulatory activity; Sulfate groups; SACCHARINA-JAPONICA; BROWN SEAWEED; STRUCTURAL CHARACTERISTICS; SULFATED POLYSACCHARIDES; EXTRACTION; HETEROPOLYSACCHARIDE; FUCOSTEROL; ALGAE;
D O I：
10.1016/j.carbpol.2024.121883
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Silvetia siliquosa, the only species of the family Fucaceae in China, is used as a medicine food homology. Fucoidan from S. siliquosa was extracted by hot water twice thoroughly (13 % of total yield), and a purified fucoidan SSF with a molecular weight of 93 kD was obtained. Chemical composition analysis demonstrated that SSF was primarily composed of sulfate (21.68 wt%) and fucose (84 % of all neutral monosaccharides). IR, methylation analysis, NMR and ESI-MS results indicated SSF had the backbone of mainly (1 -* 3)-alpha-L-fucopyranose and minor (1 -* 4)-alpha-L-fucopyranose, with little 1,3 and 1,4 branched beta-D-Xylp and beta-D-Galp. The in vitro immunomodulatory test on RAW 264.7 cells showed that SSF could up-regulate the expression of immune related factors and proteins in a concentration-dependent manner, but the immunomodulatory effect disappeared from desulfated SSF. This research indicated that highly sulfated fucan possessed immunomodulatory effect and the importance of sulfate groups in the activity of SSF.
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页数：13
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