A highly sensitive and robust one-pot assay for the detection of microRNAs

被引：1
|
作者：
Yan, He [1 ]
Zeng, Yong [1 ]
机构：
[1] Univ Florida, Dept Chem, Gainesville, FL 32611 USA
来源：
NATURE BIOMEDICAL ENGINEERING | 2023年 / 7卷 / 12期
关键词：
D O I：
10.1038/s41551-023-01043-z
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We developed exponentially amplified rolling circle amplification with CRISPR–Cas12a as a one-pot, isothermal assay for microRNA detection. This method has single-digit femtomolar sensitivity and single-nucleotide specificity, and can be deployed for point-of-care testing. The assay has been adapted for the microRNA profiling of extracellular vesicles, which is used in the diagnosis of pancreatic cancer.
引用
收藏
页码：1539 / 1540
页数：2
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