Viable tendon neotissue from adult adipose-derived multipotent stromal cells

被引:0
|
作者
Taguchi, Takashi [1 ]
Lopez, Mandi [1 ]
Takawira, Catherine [1 ]
机构
[1] Louisiana State Univ, Sch Vet Med, Vet Clin Sci Dept, Lab Equine & Comparat Orthoped Res, Baton Rouge, LA 70803 USA
基金
美国农业部;
关键词
bioengineering; ligament; stem cells; bioreactor; tissue regeneration; de novo tissue generation; equine; MESENCHYMAL STEM-CELLS; DIGITAL FLEXOR TENDINITIS; MICROFIBRILLAR COLLAGEN HEMOSTAT; TENOGENIC DIFFERENTIATION; EXTRACELLULAR-MATRIX; BONE-MARROW; THOROUGHBRED RACEHORSES; NATIONAL HUNT; GENE-EXPRESSION; FACTOR-I;
D O I
10.3389/fbioe.2023.1290693
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Tendon healing is frequently prolonged, unpredictable, and results in poor tissue quality. Neotissue formed by adult multipotent stromal cells has the potential to guide healthy tendon tissue formation.Objectives: The objective of this study was to characterize tendon neotissue generated by equine adult adipose-derived multipotent stromal cells (ASCs) on collagen type I (COLI) templates under 10% strain in a novel bioreactor. The tested hypothesis was that ASCs assume a tendon progenitor cell-like morphology, express tendon-related genes, and produce more organized extracellular matrix (ECM) in tenogenic versus stromal medium with perfusion and centrifugal fluid motion.Methods: Equine ASCs on COLI sponge cylinders were cultured in stromal or tenogenic medium within bioreactors during combined perfusion and centrifugal fluid motion for 7, 14, or 21 days under 10% strain. Viable cell distribution and number, tendon-related gene expression, and micro- and ultra-structure were evaluated with calcein-AM/EthD-1 staining, resazurin reduction, RT-PCR, and light, transmission, and scanning electron microscopy. Fibromodulin was localized with immunohistochemistry. Cell number and gene expression were compared between culture media and among culture periods (p < 0.05).Results: Viable cells were distributed throughout constructs for up to 21 days of culture, and cell numbers were higher in tenogenic medium. Individual cells had a round or rhomboid shape with scant ECM in stromal medium in contrast to clusters of parallel, elongated cells surrounded by highly organized ECM in tenogenic medium after 21 days of culture. Transcription factor, extracellular matrix, and mature tendon gene expression profiles confirmed ASC differentiation to a tendon progenitor-like cell in tenogenic medium. Construct micro- and ultra-structure were consistent with tendon neotissue and fibromodulin was present in the ECM after culture in tenogenic medium.Conclusion: Long-term culture in custom bioreactors with combined perfusion and centrifugal tenogenic medium circulation supports differentiation of equine adult ASCs into tendon progenitor-like cells capable of neotissue formation.
引用
收藏
页数:18
相关论文
共 50 条
  • [31] Adipose-derived stromal/stem cells from different adipose depots in obesity development
    Silva, Karina Ribeiro
    Baptista, Leandra Santos
    WORLD JOURNAL OF STEM CELLS, 2019, 11 (03): : 147 - 166
  • [32] Osteogenic Differentiation of Adipose-Derived Stromal Cells: From Bench to Clinics
    Kuterbekov, Mirasbek
    Jonas, Alain M.
    Glinel, Karine
    Picart, Catherine
    TISSUE ENGINEERING PART B-REVIEWS, 2020, 26 (05) : 461 - 474
  • [33] Application of Allogenic Adipose-Derived Multipotent Mesenchymal Stromal Cells from Cat for Tibial Bone Pseudoarthrosis Therapy (Case Report)
    Zakirova E.Y.
    Valeeva A.N.
    Masgutov R.F.
    Naumenko E.A.
    Rizvanov A.A.
    BioNanoScience, 2017, 7 (1) : 207 - 211
  • [34] Characterization of neuronal/glial differentiation of murine adipose-derived adult stromal cells.
    Safford, KM
    Safford, SD
    Shelly, AK
    Rice, HE
    BLOOD, 2003, 102 (11) : 180B - 180B
  • [35] In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogenesis
    Xu, Yue
    Malladi, Preeti
    Chiou, Michael
    Bekerman, Elena
    Giaccia, Amato J.
    Longaker, Michael T.
    TISSUE ENGINEERING, 2007, 13 (12): : 2981 - 2993
  • [36] Comparison of trophic factors secreted from human adipose-derived stromal vascular fraction with those from adipose-derived stromal/stem cells in the same individuals
    Hirose, Yujiro
    Funahashi, Yasuhito
    Matsukawa, Yoshihisa
    Majima, Tsuyoshi
    Yamaguchi, Masaya
    Kawabata, Shigetada
    Gotoh, Momokazu
    Yamamoto, Tokunori
    CYTOTHERAPY, 2018, 20 (04) : 589 - 591
  • [37] Potential use of human adipose-derived multipotent mesenchymal stromal cells as a drug delivery mechanism for bone engineering
    Shupletsova, V. V.
    Litvinova, L. S.
    Yurova, K. A.
    Khaziakhmatova, O. G.
    Malashchenko, V. V.
    Pokrovskaya, L.
    Kudryavtseva, V. L.
    Tverdokhlebov, S. I.
    Timin, A. S.
    Sukhorukov, G. B.
    Gow, A. J.
    Atochina-Vasserman, E.
    Khlusov, I. A.
    EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2018, 48 : 81 - 82
  • [38] Induced differentiation of adipose-derived stromal cells into myoblasts
    Guizhu Wu
    Xiu Zheng
    Zhongqing Jiang
    Jinhua Wang
    Yanfeng Song
    Journal of Huazhong University of Science and Technology [Medical Sciences], 2010, 30 : 285 - 290
  • [39] Stem molecular signature of adipose-derived stromal cells
    Peroni, Daniele
    Scambi, Ilaria
    Pasini, Annalisa
    Lisi, Veronica
    Bifari, Francesco
    Krampera, Mauro
    Rigotti, Gino
    Sbarbati, Andrea
    Galie, Mirco
    EXPERIMENTAL CELL RESEARCH, 2008, 314 (03) : 603 - 615
  • [40] Induced Differentiation of Adipose-derived Stromal Cells into Myoblasts
    Wu, Guizhu
    Zheng, Xiu
    Jiang, Zhongqing
    Wang, Jinhua
    Song, Yanfeng
    JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2010, 30 (03) : 285 - 290