Viable tendon neotissue from adult adipose-derived multipotent stromal cells

被引:0
|
作者
Taguchi, Takashi [1 ]
Lopez, Mandi [1 ]
Takawira, Catherine [1 ]
机构
[1] Louisiana State Univ, Sch Vet Med, Vet Clin Sci Dept, Lab Equine & Comparat Orthoped Res, Baton Rouge, LA 70803 USA
基金
美国农业部;
关键词
bioengineering; ligament; stem cells; bioreactor; tissue regeneration; de novo tissue generation; equine; MESENCHYMAL STEM-CELLS; DIGITAL FLEXOR TENDINITIS; MICROFIBRILLAR COLLAGEN HEMOSTAT; TENOGENIC DIFFERENTIATION; EXTRACELLULAR-MATRIX; BONE-MARROW; THOROUGHBRED RACEHORSES; NATIONAL HUNT; GENE-EXPRESSION; FACTOR-I;
D O I
10.3389/fbioe.2023.1290693
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Tendon healing is frequently prolonged, unpredictable, and results in poor tissue quality. Neotissue formed by adult multipotent stromal cells has the potential to guide healthy tendon tissue formation.Objectives: The objective of this study was to characterize tendon neotissue generated by equine adult adipose-derived multipotent stromal cells (ASCs) on collagen type I (COLI) templates under 10% strain in a novel bioreactor. The tested hypothesis was that ASCs assume a tendon progenitor cell-like morphology, express tendon-related genes, and produce more organized extracellular matrix (ECM) in tenogenic versus stromal medium with perfusion and centrifugal fluid motion.Methods: Equine ASCs on COLI sponge cylinders were cultured in stromal or tenogenic medium within bioreactors during combined perfusion and centrifugal fluid motion for 7, 14, or 21 days under 10% strain. Viable cell distribution and number, tendon-related gene expression, and micro- and ultra-structure were evaluated with calcein-AM/EthD-1 staining, resazurin reduction, RT-PCR, and light, transmission, and scanning electron microscopy. Fibromodulin was localized with immunohistochemistry. Cell number and gene expression were compared between culture media and among culture periods (p < 0.05).Results: Viable cells were distributed throughout constructs for up to 21 days of culture, and cell numbers were higher in tenogenic medium. Individual cells had a round or rhomboid shape with scant ECM in stromal medium in contrast to clusters of parallel, elongated cells surrounded by highly organized ECM in tenogenic medium after 21 days of culture. Transcription factor, extracellular matrix, and mature tendon gene expression profiles confirmed ASC differentiation to a tendon progenitor-like cell in tenogenic medium. Construct micro- and ultra-structure were consistent with tendon neotissue and fibromodulin was present in the ECM after culture in tenogenic medium.Conclusion: Long-term culture in custom bioreactors with combined perfusion and centrifugal tenogenic medium circulation supports differentiation of equine adult ASCs into tendon progenitor-like cells capable of neotissue formation.
引用
收藏
页数:18
相关论文
共 50 条
  • [21] Characterization of neuronal/glial differentiation of murine adipose-derived adult stromal cells
    Safford, KM
    Safford, SD
    Gimble, JM
    Shetty, AK
    Rice, HE
    EXPERIMENTAL NEUROLOGY, 2004, 187 (02) : 319 - 328
  • [22] Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells
    Lu, Yanhui
    Yuan, Xiaodong
    Sun, Qiaoyu
    Ou, Ya
    NEURAL REGENERATION RESEARCH, 2013, 8 (10) : 882 - 889
  • [23] Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells
    Yanhui Lu
    Xiaodong Yuan
    Qiaoyu Sun
    Ya Ou
    NeuralRegenerationResearch, 2013, 8 (10) : 882 - 889
  • [24] Angiogenic potential of spheroids from umbilical cord and adipose-derived multipotent mesenchymal stromal cells within fibrin gel
    Gorkun, A. A.
    Shpichka, A., I
    Zurina, I. M.
    Koroleva, A., V
    Kosheleva, N., V
    Nikishin, D. A.
    Butnaru, D., V
    Timashev, P. S.
    Repin, V. S.
    Saburina, I. N.
    BIOMEDICAL MATERIALS, 2018, 13 (04)
  • [25] Age-related ultrastructural changes in spheroids of the adipose-derived multipotent mesenchymal stromal cells from ovariectomized mice
    Kyryk, Vitalii
    Tsupykov, Oleg
    Ustymenko, Alina
    Smozhanik, Ekaterina
    Govbakh, Iryna
    Butenko, Gennadii
    Skibo, Galyna
    FRONTIERS IN CELLULAR NEUROSCIENCE, 2023, 17
  • [26] Adipose tissue engineering by human adipose-derived stromal cells
    Hong, Liu
    Peptan, Ioana A.
    Colpan, Aylin
    Daw, Joseph L.
    CELLS TISSUES ORGANS, 2006, 183 (03) : 133 - 140
  • [27] Adipose-derived stromal vascular fraction improves tendon healing in rabbits
    Mehdi Behfar
    Farshid Sarrafzadeh-Rezaei
    Rahim Hobbenaghi
    Nowruz Delirezh
    Bahram Dalir-Naghadeh
    中华创伤杂志(英文版), 2011, (06) : 329 - 335
  • [28] Adipose-derived stromal vascular fraction improves tendon healing in rabbits
    Behfar, Mehdi
    Sarrafzadeh-Rezaei, Farshid
    Hobbenaghi, Rahim
    Delirezh, Nowruz
    Dalir-Naghadeh, Bahram
    CHINESE JOURNAL OF TRAUMATOLOGY, 2011, 14 (06) : 329 - 335
  • [29] Silk fibroin induces chondrogenic differentiation of canine adipose-derived multipotent mesenchymal stromal cells/mesenchymal stem cells
    Voga, Metka
    Drnovsek, Natasa
    Novak, Sasa
    Majdic, Gregor
    JOURNAL OF TISSUE ENGINEERING, 2019, 10
  • [30] Hematopoiesis in bone marrow regenerated from adipose-derived stromal cells
    Migita, Makoto
    Fujita, Atsushi
    Ogawa, Rei
    Ueda, Takabiro
    Fukunaga, Yoshitaka
    Shimada, Takashi
    BLOOD, 2007, 110 (11) : 96B - 96B