Evaluation of BACTEC MGIT 960 system for recovery of Nocardia from clinical specimens

被引：5

作者：

Hu, Yanning ^{[1
,2
,3
]}

Zhu, Yue ^{[2
,3
]}

Li, Chen ^{[1
,2
,3
,6
]}

Shi, Huihui ^{[7
]}

Zhang, Yulin ^{[2
,3
]}

Zhao, Jiankang ^{[2
,3
]}

Fan, Yanyan ^{[2
,3
]}

Wu, Yongli ^{[2
,3
,4
]}

Li, Ziyao ^{[2
,3
,5
]}

Liu, Xinmeng ^{[1
,2
,3
]}

Zhang, Feilong ^{[1
,2
,3
]}

Lu, Binghuai ^{[1
,2
,3
,4
,5
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Peking Univ China Japan Friendship Sch Clin Med, Beijing, Peoples R China

[2] China Japan Friendship Hosp, Natl Ctr Resp Med, Dept Pulm & Crit Care Med, Lab Clin Microbiol & Infect Dis, Beijing, Peoples R China

[3] Chinese Acad Med Sci, Inst Resp Med, Beijing, Peoples R China

[4] Chinese Acad Med Sci, Peking Union Med Coll, Beijing, Peoples R China

[5] China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China

[6] Liuyang Tradit Chinese Med Hosp, Dept Clin Lab, Changsha, Hunan, Peoples R China

[7] Shanghai Univ, Affiliated Nantong Hosp, Dept Clin Lab, Nantong, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 106卷 / 04期

关键词：

Nocardia; Nocardiosis; BACTEC MGIT 960; Smear microscopy; Nocardia farcinica; SUSCEPTIBILITY;

D O I：

10.1016/j.diagmicrobio.2023.115989

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Nocardia spp. is an aerobic Gram-positive bacillus responsible for nocardiosis. Herein, we performed a retro-spective study to evaluate the performance of BACTEC MGIT 960 system, in comparison with smear microscopy and blood agar plate (BAP) culture, to recover Nocardia from different clinical specimens. Furthermore, the inhibitory effect of antibiotics contained in MGIT 960 tube on Nocardia was also evaluated. The sensitivities for Nocardia recovery using smear microscopy, BAP culture, and MGIT 960 were 39.4% (54/137), 46.1% (99/215), and 81.3% (156/192), respectively. N. farcinica was the most detected species (60.4%, 136/225). In MGIT 960-recovered Nocardia strains, N. farcinica accounted for 76.9%. Furthermore, trimethoprim in MGIT 960 tube inhib-ited less N. farcinica growth than that of other Nocardia species, partially explaining why MGIT 960 recovered more N. farcinica from sputa. The current study demonstrated that MGIT 960 could recover Nocardia strains from heavily-contaminated samples if its components and antibiotics are redesigned.(c) 2023 Elsevier Inc. All rights reserved.

引用

页数：7

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