Electrolysis of Bacteria Based on Microfluidic Technology

被引:3
|
作者
Zhao, Jianqiu [1 ]
Li, Na [1 ]
Zhou, Xinyu [1 ]
Yu, Zihan [1 ]
Lan, Mei [1 ]
Chen, Siyu [1 ]
Miao, Jiajia [1 ]
Li, Yulai [1 ]
Li, Guiying [1 ]
Yang, Fang [1 ]
机构
[1] Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China
关键词
cell lysis; E; coli; microfluidic; AC electric field; electroporation; CELL-LYSIS; CHIP; ARRAYS; DEVICE;
D O I
10.3390/mi14010144
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cell lysis is a key step for studying the structure and function of proteins in cells and an important intermediate step in drug screening, cancer diagnosis, and genome analysis. The current cell lysis methods still suffer from limitations, such as the need for large instruments, a long and time-consuming process, a large sample volume, chemical reagent contamination, and their unsuitability for the small amount of bacteria lysis required for point-of-care testing (POCT) devices. Therefore, a fast, chemical-free, portable, and non-invasive device needs to be developed. In the present study, we designed an integrated microfluidic chip to achieve E. coli lysis by applying an alternating current (AC) electric field and investigated the effects of voltage, frequency, and flow rate on the lysis. The results showed that the lysis efficiency of the bacteria was increased with a higher voltage, lower frequency, and lower flow rate. When the voltage was at 10 Vp-p, the lysis efficiency was close to 100%. The study provided a simple, rapid, reagent-free, and high-efficiency cleavage method for biology and biomedical applications involving bacteria lysis.
引用
收藏
页数:7
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