Electrolysis of Bacteria Based on Microfluidic Technology

被引:3
|
作者
Zhao, Jianqiu [1 ]
Li, Na [1 ]
Zhou, Xinyu [1 ]
Yu, Zihan [1 ]
Lan, Mei [1 ]
Chen, Siyu [1 ]
Miao, Jiajia [1 ]
Li, Yulai [1 ]
Li, Guiying [1 ]
Yang, Fang [1 ]
机构
[1] Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China
关键词
cell lysis; E; coli; microfluidic; AC electric field; electroporation; CELL-LYSIS; CHIP; ARRAYS; DEVICE;
D O I
10.3390/mi14010144
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cell lysis is a key step for studying the structure and function of proteins in cells and an important intermediate step in drug screening, cancer diagnosis, and genome analysis. The current cell lysis methods still suffer from limitations, such as the need for large instruments, a long and time-consuming process, a large sample volume, chemical reagent contamination, and their unsuitability for the small amount of bacteria lysis required for point-of-care testing (POCT) devices. Therefore, a fast, chemical-free, portable, and non-invasive device needs to be developed. In the present study, we designed an integrated microfluidic chip to achieve E. coli lysis by applying an alternating current (AC) electric field and investigated the effects of voltage, frequency, and flow rate on the lysis. The results showed that the lysis efficiency of the bacteria was increased with a higher voltage, lower frequency, and lower flow rate. When the voltage was at 10 Vp-p, the lysis efficiency was close to 100%. The study provided a simple, rapid, reagent-free, and high-efficiency cleavage method for biology and biomedical applications involving bacteria lysis.
引用
收藏
页数:7
相关论文
共 50 条
  • [41] An Improved Multifunctional Frequency Selective Surface based on Microfluidic Technology
    Ghosh, Saptarshi
    Phon, Ratanak
    Tentzeris, Manos M.
    Lim, Sungjoon
    2018 INTERNATIONAL SYMPOSIUM ON ANTENNAS AND PROPAGATION (ISAP), 2018,
  • [42] Terahertz spectral characteristics of photosensitive resin based on microfluidic technology
    Zhang, Xin-Rui
    Yang, Hui-Yu
    Peng, Zhuang
    Su, Bo
    Zhang, Cun-Lin
    AIP ADVANCES, 2024, 14 (02)
  • [43] Multifunctional Micro/Nanoscale Fibers Based on Microfluidic Spinning Technology
    Du, Xiang-Yun
    Li, Qing
    Wu, Guan
    Chen, Su
    ADVANCED MATERIALS, 2019, 31 (52)
  • [44] Synthesis and Surface Engineering of Inorganic Nanomaterials Based on Microfluidic Technology
    Shen, Jie
    Shafiq, Muhammad
    Ma, Ming
    Chen, Hangrong
    NANOMATERIALS, 2020, 10 (06) : 1 - 29
  • [45] Advances in Droplet-Based Microfluidic Technology and Its Applications
    Liu Zhao-Miao
    Yang Yang
    Du Yu
    Pang Yan
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2017, 45 (02) : 282 - 295
  • [46] A Microfluidic Interposer Based on Three Dimensional Molded Substrate Technology
    Leneke, T.
    Majcherek, S.
    Hirsch, S.
    Schmidt, M-P.
    Schmidt, B.
    2014 INTERNATIONAL CONFERENCE ON ELECTRONICS PACKAGING (ICEP), 2014, : 223 - 226
  • [47] Reviews on the Structure and Morphology Control of Explosives Based on Microfluidic Technology
    Shi J.
    Zhu P.
    Shen R.
    Hanneng Cailiao/Chinese Journal of Energetic Materials, 2022, 30 (05): : 511 - 526
  • [48] Fabrication of microelectrodes based on microfluidic pulse inertial jetting technology
    Yang, Li-Jun
    Zhu, Li
    Zhu, Xiao-Yang
    Lu, Bao-Chun
    Wang, Hong-Cheng
    Zhang, Wei-Yi
    Guangxue Jingmi Gongcheng/Optics and Precision Engineering, 2015, 23 : 291 - 296
  • [49] Microfluidic chip architectures for a cell sorter based on the electrowetting technology
    Schreiber, Fedor
    Kahnert, Stefan
    Goehlich, Andreas
    Greifendorf, Dieter
    Bartels, Frank
    Janzyk, Udo
    Lennartz, Klaus
    Kirstein, Uwe
    Rennings, Andreas
    Kueppers, Ralf
    Erni, Daniel
    TM-TECHNISCHES MESSEN, 2016, 83 (05) : 274 - 288
  • [50] Microfluidic-based isolation of bacteria from whole blood for sepsis diagnostics
    Zelenin, Sergey
    Hansson, Jonas
    Ardabili, Sahar
    Ramachandraiah, Harisha
    Brismar, Hjalmar
    Russom, Aman
    BIOTECHNOLOGY LETTERS, 2015, 37 (04) : 825 - 830