Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish

被引：3

作者：

Chopra, Kunal ^{[1
]}

Folkmanait, Milda ^{[1
,4
]}

Stockdale, Liam ^{[1
]}

Shathish, Vishali ^{[2
]}

Ishibashi, Shoko ^{[1
]}

Bergin, Rachel ^{[1
]}

Amich, Jorge ^{[2
,3
]}

Amaya, Enrique ^{[1
]}

机构：

[1] Univ Manchester, Fac Biol, Sch Biol Sci, Div Cell Matrix Biol & Regenerat Med, Manchester M13 9PT, England

[2] Univ Manchester, Fac Biol, Sch Biol Sci, Div Evolut, Manchester M13 9PT, England

[3] Inst Salud Carlos III ISCIII, Mycol Reference Lab, Natl Ctr Microbiol, Madrid 28220, Spain

[4] Univ Oxford, Dept Physiol & Anat & Genet, Oxford QK1 3PT, England

来源：

ISCIENCE | 2023年 / 26卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

CHRONIC GRANULOMATOUS-DISEASE; ENCODING P22(PHOX); CIRCADIAN CLOCK; INFECTIONS; PHYSIOLOGY; CAPACITY; DEFECTS; INJURY;

D O I：

10.1016/j.isci.2023.106147

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sustained elevated levels of reactive oxygen species (ROS) have been shown to be essential for regeneration in many organisms. This has been shown primarily via the use of pharmacological inhibitors targeting the family of NADPH oxidases (NOXes). To identify the specific NOXes involved in ROS production during adult caudal fin regeneration in zebrafish, we generated nox mutants for duox, nox5 and cyba (a key subunit of NOXes 1-4) and crossed these lines with a transgenic line ubiquitously expressing HyPer, which permits the measurement of ROS levels. Homozygous duox mutants had the greatest effect on ROS levels and rate of fin regeneration among the single mutants. However, duox: cyba double mutants showed a greater effect on fin regeneration than the single duox mutants, suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm.

引用

页数：21

共 27 条

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