Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9

被引：1

作者：

Maierhof, Smilla K. ^{[1
,2
,3
,4
,5
]}

Schinke, Christian ^{[1
,2
,3
,4
]}

Cernoch, Janine ^{[6
]}

Hew, Lois ^{[1
,2
,3
,5
]}

Bruske, Laura Pauline ^{[7
]}

Vallone, Valeria Fernandez ^{[6
]}

Fischer, Kristin ^{[6
]}

Stachelscheid, Harald ^{[6
,8
]}

Huehnchen, Petra ^{[1
,2
,3
,4
,9
,10
,11
]}

Endres, Matthias ^{[1
,2
,3
,4
,5
,9
,10
,11
,12
,13
,14
]}

Diecke, Sebastian ^{[15
,16
]}

Telugu, Narasimha Swamy ^{[15
]}

Boehmerle, Wolfgang ^{[1
,2
,3
,4
,9
,10
,11
]}

机构：

[1] Charite Univ Med Berlin, Berlin, Germany

[2] Free Univ Berlin, Berlin, Germany

[3] Humboldt Univ, Klin & Hochschulambulanz Neurol, Berlin, Germany

[4] Univ Med Berlin, Berlin Inst Hlth Charite, Anna Louisa Karsch Str 2, D-10178 Berlin, Germany

[5] Univ Med Berlin, Einstein Ctr Neurosci Charite, Charitepl 1, D-10117 Berlin, Germany

[6] Charite Univ Med Berlin, Core Unit Pluripotent Stem Cells & Organoids CUSCO, Berlin Inst Hlth, Charitepl 1, D-10117 Berlin, Germany

[7] Berlin Univ Appl Sci & Technol BHT, Luxemburger Str 10, D-13353 Berlin, Germany

[8] Charite Univ Med Berlin, BIH Ctr Regenerat Therapies BCRT, Berlin Inst Hlth BIH, Charitepl 1, D-10117 Berlin, Germany

[9] Charite Univ Med Berlin, Charitepl 1, D-10117 Berlin, Germany

[10] Free Univ Berlin, Charitepl 1, D-10117 Berlin, Germany

[11] Humboldt Univ, NeuroCure Cluster Excellence, Charitepl 1, D-10117 Berlin, Germany

[12] Charite Univ Med Berlin, Ctr Stroke Res, Charitepl 1, D-10117 Berlin, Germany

[13] German Ctr Neurodegenerat Dis DZNE, Partner Site Berlin, Berlin, Germany

[14] German Ctr Cardiovasc Res DZHK, Partner Site Berlin, Berlin, Germany

[15] Max Delbruck Ctr Mol Med Helmholtz Assoc MDC, Technol Platform Pluripotent Stemcell, D-13125 Berlin, Germany

[16] German Ctr Cardiovasc Res DZHK, Partner Site Berlin, Berlin, Germany

来源：

STEM CELL RESEARCH | 2023年 / 73卷

关键词：

D O I：

10.1016/j.scr.2023.103253

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease.

引用

页数：6

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