Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9

被引：1
|
作者：
Maierhof, Smilla K. [1 ,2 ,3 ,4 ,5 ]
Schinke, Christian [1 ,2 ,3 ,4 ]
Cernoch, Janine [6 ]
Hew, Lois [1 ,2 ,3 ,5 ]
Bruske, Laura Pauline [7 ]
Vallone, Valeria Fernandez [6 ]
Fischer, Kristin [6 ]
Stachelscheid, Harald [6 ,8 ]
Huehnchen, Petra [1 ,2 ,3 ,4 ,9 ,10 ,11 ]
Endres, Matthias [1 ,2 ,3 ,4 ,5 ,9 ,10 ,11 ,12 ,13 ,14 ]
Diecke, Sebastian [15 ,16 ]
Telugu, Narasimha Swamy [15 ]
Boehmerle, Wolfgang [1 ,2 ,3 ,4 ,9 ,10 ,11 ]
机构：
[1] Charite Univ Med Berlin, Berlin, Germany
[2] Free Univ Berlin, Berlin, Germany
[3] Humboldt Univ, Klin & Hochschulambulanz Neurol, Berlin, Germany
[4] Univ Med Berlin, Berlin Inst Hlth Charite, Anna Louisa Karsch Str 2, D-10178 Berlin, Germany
[5] Univ Med Berlin, Einstein Ctr Neurosci Charite, Charitepl 1, D-10117 Berlin, Germany
[6] Charite Univ Med Berlin, Core Unit Pluripotent Stem Cells & Organoids CUSCO, Berlin Inst Hlth, Charitepl 1, D-10117 Berlin, Germany
[7] Berlin Univ Appl Sci & Technol BHT, Luxemburger Str 10, D-13353 Berlin, Germany
[8] Charite Univ Med Berlin, BIH Ctr Regenerat Therapies BCRT, Berlin Inst Hlth BIH, Charitepl 1, D-10117 Berlin, Germany
[9] Charite Univ Med Berlin, Charitepl 1, D-10117 Berlin, Germany
[10] Free Univ Berlin, Charitepl 1, D-10117 Berlin, Germany
[11] Humboldt Univ, NeuroCure Cluster Excellence, Charitepl 1, D-10117 Berlin, Germany
[12] Charite Univ Med Berlin, Ctr Stroke Res, Charitepl 1, D-10117 Berlin, Germany
[13] German Ctr Neurodegenerat Dis DZNE, Partner Site Berlin, Berlin, Germany
[14] German Ctr Cardiovasc Res DZHK, Partner Site Berlin, Berlin, Germany
[15] Max Delbruck Ctr Mol Med Helmholtz Assoc MDC, Technol Platform Pluripotent Stemcell, D-13125 Berlin, Germany
[16] German Ctr Cardiovasc Res DZHK, Partner Site Berlin, Berlin, Germany
来源：
STEM CELL RESEARCH | 2023年 / 73卷
关键词：
D O I：
10.1016/j.scr.2023.103253
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease.
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页数：6
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